新型壳寡糖γ-氨基丁酸衍生物的制备及其对小麦幼苗抗旱作用的研究  

Preparation of novelγ-aminobutyric acid-modified chitooligosaccharide and their effects on drought resistance of wheat seedlings

作　　者：尹秀晶 刘松[1,2,3] 秦玉坤 邢荣娥[1,2,3] 于春林[1,2,3,4] 李克成 李鹏程[1,2,3] YIN Xiu-jing;LIU Song;QIN Yu-kun;XING Rong-e;YU Chun-lin;LI Ke-cheng;LI Peng-cheng(Key Laboratory of Experimental Marine Biology,titute of Oceanology of the Chinese Academy of Sciences,Qingdao 266071;Functional Laboratory of Marine Drugs and Biological Products,Pilot National Laboratory of Marine Science and Technology(Qingdao),Qingdao 266237,China;Center for Ocean Mega-science,Chinese Academy of Sciences,Qingdao 266071,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237 [3]中国科学院海洋大科学研究中心,山东青岛266071 [4]中国科学院大学,北京100049

出　　处：《海洋科学》2020年第5期42-52,共11页Marine Sciences

基　　金：山东省重点研发项目(2018GHY115008,2019GHY112015,2018GHY115017)。

摘　　要：利用壳寡糖氨基与γ-氨基丁酸羧基发生酰胺化反应合成新型壳寡糖-氨基丁酸衍生物,并通过傅里叶变换红外光谱(FT-IR)、核磁共振光谱(NMR)等对合成的衍生物进行结构表征。以小麦种子为材料,各样品对小麦种子进行浸种处理后干旱胁迫培养到两叶一心,测定小麦生理生化指标,评价新型衍生物的抗旱作用。结果表明,新型壳寡糖γ-氨基丁酸衍生物成功合成,并且对干旱胁迫下小麦生理生化指标有明显的影响,衍生物明显降低了小麦幼苗叶片的丙二醛含量和细胞膜透性,缓解了干旱胁迫对小麦细胞膜的损伤,增加可溶性糖含量进而增强小麦植物对土壤中水分的吸收能力,减少了干旱胁迫对生理代谢的影响。总之,成功合成了具有较强诱导小麦抗旱作用的新型壳寡糖γ-氨基丁酸衍生物,为新型植物抗逆诱导剂的开发提供了思路。The novelγ-aminobutyric acid-modified chitooligosaccharide was synthesized by the amination of the amino group of the chitooligosaccharide with the carboxyl group ofγ-aminobutyric acid,and its structure was characterized using Fourier-transform infrared and magnetic resonance spectra(NMR).In this paper,wheat seeds were soaked with chitooligosaccharide,γ-aminobutyric acid,andγ-aminobutyric acid-modified chitooligosaccharide.Their drought-resistant effects were evaluated by measuring the relevant indicators after they grew for a two-leave period under drought stress.The results showed that the novel derivatives were synthesized successfully and had positive effects on wheat under drought stress.The derivatives significantly reduced the malondialdehyde content and cell membrane permeability of wheat seedling leaves,alleviated the damage to the wheat cell membrane caused by drought stress,increased the soluble sugar content,and enhanced the soil water absorption capacity of wheat plants.Therefore,the newly synthesizedγ-aminobutyric acid-modified chitooligosaccharide can significantly reduce the effects of drought stress on wheat metabolism.In conclusion,a novel chitooligosaccharide derivative with a robust drought-induced effect on wheat was successfully synthesized and might provide an idea for the development of a new plant stress-resistant inducer.

关 键 词：壳寡糖 Γ-氨基丁酸 抗旱 

分 类 号：Q945[生物学—植物学]