废电池污染土壤中重金属对小鼠的毒性效应  

The Toxic Effect of Heavy Metals from Soil Contaminated by Waste Batteries on Mice

作　　者：王文倩 陈浩浩[1] 傅晓艳[1] 蒋进展[1] 潘晓明[1] 张志琴[1] WANG Wenqian;CHEN Haohao;FU Xiaoyan;JIANG Jinzhan;PAN Xiaoming;ZHANG Zhiqin(Jinhua Polytechnic,Jinhua 321007,China)

机构地区：[1]金华职业技术学院,浙江金华321007

出　　处：《金华职业技术学院学报》2020年第3期83-88,共6页Journal of Jinhua Polytechnic

基　　金：浙江省大型仪器检测分析项目(2018C37074);浙江省2018年度高校访问工程师“校企合作项目”(FG2018029)。

摘　　要：目的探索废电池造成的土壤重金属污染对小鼠肝脏的毒性效应。方法将21只1月龄ICR小鼠,按体重随机分为三组,每组7只。其中一组饲喂普通饲料,一组饲喂含有未污染土壤的饲料,一组饲喂含有废电池污染土壤的饲料。饲喂10天后结束,称量小鼠体重,并测定肝脏组织中重金属含量,观察肝脏组织学变化,测定肝脏组织中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量,检测肝脏组织中金属硫蛋白(MT1和MT2)的mRNA表达水平。结果与对照组相比,废电池引起的污染土壤减少了小鼠29%的体重,提升小鼠肝脏内30%的MDA,并引起肝脏炎症浸润、细胞核固缩等组织损伤。与此同时,废电池污染土壤组中的小鼠显著提高MT1和MT2基因的表达应对重金属的胁迫。结论废电池造成的土壤污染对小鼠肝脏具有损伤,具有潜在的健康风险。In order to better understand the toxic effect of metal pollution in soil caused by waste batteries, the mice were selected as experimental subjects to assess the toxic effect of metal via feeding food containing polluted soil caused by waste batteries. After 10 days, mice weight, superoxide dismutase(SOD) activity, malondialdehyde(MDA) levels, histological change and the transcriptional levels of metallothionein(MT) were measured to assess the health risk of polluted soil caused by waste batteries.Our result clearly indicated that compared with control group polluted soil by waste batteries decreased mice weight by 29%, increased MDA levels by 30%, and caused damage on the liver tissues. Meanwhile,MT1 and MT2 mRNA transcriptional levels were significantly elevated by 120, 300% to deal with stress caused by metals. Soil pollution caused by waste batteries damaged the liver of mice and posed a potential health risk.

关 键 词：废电池 重金属 小鼠 氧化损伤 组织损伤 基因调控 

分 类 号：R124.3[医药卫生—环境卫生学]