生长调节剂对‘香妃’含笑扦插生根及相关酶活性的影响  

Effects of growth regulators on rooting and correlative enzyme activities of Michelia figo ‘Xiangfei’ cuttings

作　　者：郑巧巧 兰思仁[1,2] 刘雪蝶 冯玉超 彭东辉[2] ZHENG Qiaoqiao;LAN Siren;LIU Xuedie;FENG Yuchao;PENG Donghui(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Ornamental Plant Germplasm Resources Innovation&Engineering Application Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学福建省观赏植物种质资源创新与应用工程技术研究中心,福建福州350002

出　　处：《中南林业科技大学学报》2020年第5期67-76,共10页Journal of Central South University of Forestry & Technology

基　　金：国家科技支撑计划项目(2014BAD15B00);国家林业局森林公园工程技术研究中心资助项目(PTJH15002);福建省观赏植物种质资源创新与应用工程技术研究中心资助项目(PTJH16005)。

摘　　要：【目的】解决新品种‘香妃’含笑生根困难、育苗时间长的扦插繁殖问题,为该种系统扦插繁殖技术的建立、后期快繁技术的不断创新和生产实践应用提供理论依据。【方法】以‘香妃’含笑为试验材料,研究了K-IBA和GGR62种生长调节剂(200、500、800 mg·L^-1)对扦插生根的影响以及生根过程中过氧化物酶(POD)、吲哚乙酸氧化酶(IAAO)和多酚氧化酶(PPO)3种酶活性的动态变化。【结果】根据形态学观察初步判定,‘香妃’含笑插穗生根既有皮部生根型,又有愈伤组织生根型,且皮部生根与愈伤组织生根是相互抑制的,同时适宜质量浓度的生长调节剂在某种程度上可改变其生根方式。2种生长调节剂各水平在6个指标中基本均以200 mg·L^-1浸泡2 h效果最佳,且K-IBA 200 mg·L^-1平均根长、生根率和最长根长均显著高于清水处理(CK);经相关分析和主成分分析得出,7个处理综合评价值前4名排序为K-IBA 200 mg·L^-1>CK>K-IBA 500 mg·L^-1>GGR6200 mg·L^-1,K-IBA在一定质量浓度的施用下有利于插穗生根和改善根系质量。在扦插生根过程中3种酶活性变化规律各不相同,POD活性变化曲线为双峰型,K-IBA可使POD活性双峰特性明显且提前发生,也可降低POD和IAAO活性,促进皮部皮孔开裂与愈伤组织诱导,从而提高生根率,促进根系发育;PPO活性的高低与扦插生根进程有关,且受K-IBA生长调节剂的影响,同时愈伤组织生根需要依靠PPO产生更多的辅助生根因子。【结论】在本试验条件下,‘香妃’含笑选用K-IBA 200 mg·L^-1处理,生根率达75.83%,比清水处理提高了14.99%,且K-IBA的施用可以显著改变酶活性,使其共同向促进生根的方向发生着相对应的变化。鉴于本试验K-IBA生长调节剂质量浓度所设梯度较大,200 mg·L^-1是否为提高扦插生根的最佳处理还需做进一步的研究。【Objective】In order to solve the cutting propagation problem of the new variety of ‘Xiangfei’ with difficultly taking root and long seedling-raising time, which has not yet been reported up to now, and also provide a theoretical basis for the establishment of systematic cutting propagation technology of this tree species, the continuous innovation of rapid propagation technology in the later period and the research on the application of production practice.【Method】With ‘Xiangfei’ as the experimental material in this study, studied the effects of 200, 500, 800 mg·L^-1 concentration of K-IBA & GGR6 growth regulators on the rooting of cuttings, and dynamic changes of three enzyme activities during cutting rooting: peroxidase(POD), indoleacetic acid oxidase(IAAO) & polyphenol oxidase(PPO).【Result】According to the morphological observation, preliminarily determined that the rooting type of ‘Xiangfei’ cuttings included both cortex rooting and callus rooting, in this research found also cortex rooting & callus rooting were mutually inhibited, meanwhile the suitable concentration of growth regulator could change rooting style to some extent. Among the six indicators, 200 mg·L^-1 soaking for 2 h was the best for each level of the two growth regulators, the average root length, rooting rate & longest root length of K-IBA 200 mg·L^-1 were significantly higher than that of clear water treatment(CK);the correlation analysis and principal component analysis showed that the top four rankings of the seven treatments were K-IBA 200 mg·L^-1> CK > K-IBA 500 mg·L^-1> GGR6 200 mg·L^-1, K-IBA was beneficial to cuttings rooting and improving quality of root system under a certain concentration of application. The change regularities of three enzyme activities were different during cutting rooting: K-IBA could make POD activity bimodal characteristic obvious & occur in advance, and can also reduce the POD activity & IAAO activity to promote the cracking of cortex & the induction of callus, thus increasing t

关 键 词：‘香妃’含笑 生长调节剂 扦插生根 过氧化物酶 吲哚乙酸氧化酶 多酚氧化酶 

分 类 号：S718.43[农业科学—林学]