甘蓝型油菜BnaA06.mTERF1基因的功能及进化分析  

Functional and evolutional analysis of BnaA06.mTERF1 gene in Brassica napus L.

作　　者：李双 叶姜[2] 詹杰鹏[2] 朱瑶瑶 危文亮 师家勤[2] 王新发[2] LI Shuang;YE Jiang;ZHAN Jie-peng;ZHU Yao-yao;WEI Wen-liang;SHI Jia-qin;WANG Xin-fa(College of Agriculture,Yangtze University,Hubei Collaborative Innovation Center for Grain Industry,Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Jingzhou 434025,China;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China)

机构地区：[1]长江大学农学院,主要粮食作物产业化湖北省协同创新中心,湿地生态与农业利用教育部工程研究中心,湖北荆州434025 [2]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《中国油料作物学报》2020年第2期230-239,共10页Chinese Journal of Oil Crop Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(1610172017001);现代农业产业技术体系建设专项资金(CARS-13);中国农业科学院科技创新工程(CAAS-ASTIP-2013-OCRI)。

摘　　要：在高等植物中,线粒体转录终止因子(mTERF)影响线粒体或叶绿体的基因表达及植物对逆境的反应等生物学过程。为了研究甘蓝型油菜中线粒体转录终止因子1(mTERF1)的功能,本研究以甘蓝型油菜品种中双11号为材料,在A06连锁群上克隆了mTERF1,该基因编码285个氨基酸,含有5个mTERF功能结构域。利用生物信息学分析筛选到37个甘蓝型油菜mTERF家族蛋白,并进行了系统进化树、基因结构及染色体定位分析。此外,构建甘蓝型油菜BnaA06.mTERF1基因的功能互补表达载体,转化拟南芥mTERF1(soldat10)突变体后,拟南芥的表型恢复到野生型(Ler),说明该基因在油菜和拟南芥中具有保守的功能。本研究填补了油菜中线粒体转录终止因子研究的空白,为进一步探究油菜中线粒体转录终止因子的分子功能奠定了基础。In higher plants, mitochondrial transcription termination factors(mTERF) influence mitochondria or chloroplast gene expression, plant responses to stress and so on. In order to study the function of mitochondrial transcription termination factor 1(mTERF1) in Brassica napus(rapeseed), mTERF1 was cloned from Zhongshuang 11 on A06 linkage group BnaA06.mTERF1, which encoded 285 amino acids and 5 mTERF functional domains. Thirty seven mTERF family proteins were screened by bioinformatics method. Their phylogenetic tree, gene structure and chromosome location were further analyzed. In addition, the plant functional expression vector of BnaA06.mTERF1 was constructed and transformed into Arabidopsis mutant soldat10. The phenotype of the mutant recovered to wild type(Ler), indicating a conserved function of BnaA06.mTERF1 in rapeseed and Arabidopsis. This paper filled the gap in study of mitochondrial transcription termination factors in rapeseed and laid a foundation for further identification on molecular functions of mitochondrial transcription termination factors.

关 键 词：甘蓝型油菜 mTERF1 基因克隆 进化关系 

分 类 号：S565.4[农业科学—作物学]