乙型肝炎病毒PreS1抗原及e抗原表达与血清乙型肝炎病毒DNA表达载量的关系  

Relationship between expression of PreS1 antigen and e antigen with serum hepatitis B virus DNA expression load

作　　者：戴平 DAI Ping(Department of Clinical Laboratory,Lixian County People′s Hospital,Changde,Hunan 415500,China)

机构地区：[1]湖南省常德市澧县人民医院检验科,湖南常德415500

出　　处：《检验医学与临床》2020年第10期1384-1386,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨乙型肝炎病毒(HBV)前S1抗原(PreS1-Ag)及HBV e抗原(HBeAg)的表达与产妇血清HBV-DNA载量的关系。方法选取2017年3月至2019年3月于该院诊治的乙型肝炎(简称乙肝)产妇132例作为研究对象,均经临床诊断确诊为HBV感染。采用ELISA检测HBeAg、PreS1-Ag;采用实时荧光定量PCR技术定量检测HBV-DNA;根据HBV-DNA载量分组,比较各组HBeAg、PreS1-Ag检出率,计算HBeAg、PreS1-Ag检测的灵敏度及特异度。结果132例乙肝患者中,HBV-DNA载量<10^3 copy/mL有73例,其中PreS1-Ag阳性39例(53.42%),HBeAg阳性5例(6.85%);HBV-DNA载量在10^3~10^5 copy/mL有21例,其中PreS1-Ag阳性11例(52.38%),HBeAg阳性8例(38.10^%);HBV-DNA载量>10^5~10^8 copy/mL有33例,其中PreS1-Ag阳性29例(87.88%),HBeAg阳性21例(63.64%);HBV-DNA载量>10^8 copy/mL有5例,其中PreS1-Ag和HBeAg的检出率均为10^0.00%。以HBV-DNA载量为10^3 copy/mL为切割值,PreS1-Ag和HBeAg的特异度分别为69.39%、72.34%,灵敏度分别为53.01%、86.84%。结论随着HBV-DNA载量的升高,PreS1-Ag和HBeAg的检出率大幅提高,且PreS1-Ag的诊断灵敏度高于HBeAg;而与PreS1-Ag相比,HBeAg的诊断特异度更高。Objective To investigate the relationship between the expressions of PreS1 antigen(PreS1-Ag)and e antigen(HBeAg)with the load of serum hepatitis B virus DNA(HBV-DNA)in parturients.Methods One hundred and thirty-two parturients with hepatitis B diagnosed and treated in this hospital were selected as the study subjects.All cases were diagnosed as hepatitis B virus(HBV)infection by clinic.The levels of HBeAg and PreS1-Ag were detected by ELISA.The HBV-DNA load was detected by real-time fluorescence quantitative PCR.The grouping was performed according to the HBV-DNA load.The detection rates of HBeAg and PreS1-Ag were compared among the groups.The sensitivity and specificity of HBeAg and PreS1-Ag were calculated.Results Among 132 cases of hepatitis B,there were 73 cases of HBV-DNA load<103 copy/mL,including 39 cases(53.42%)of PreS1-Ag positive and 5 cases(6.85%)of HBeAg positive;in 21 cases of HBVDNA load 10^3-10^5 copy/mL,11 cases(52.38%)were PreS1-Ag positive,8 cases(38.10%)were HBeAg positive,in 33 cases of HBV-DNA load>10^5-10^8 copy/mL,29 cases(87.88%)were PreS1-Ag positive and 21 cases(63.64%)were HBeAg positive;in 5 cases of HBV-DNA load >10^8 copy/mL,the detection rates of PreS1-Ag and HBeAg all were 100.00%.With the HBV-DNA load 10^3 copy/mL as the cut off value,the specificities of PreS1-Ag and HBeAg were 69.39%and 72.34%respectively,the sensitivities were 53.01%and 86.84%respectively.Conclusion With the increase of HBV-DNA load,the detection rates of PreS1-Ag and HBeAg are increased greatly,moreover the diagnostic sensitivity of PreS1-Ag is higher than that of HBeAg;but compared with PreS1-Ag,the diagnostic specificity of HBeAg is higher.

关 键 词：乙肝病毒 PRES1抗原 乙型肝炎病毒E抗原 HBV-DNA 

分 类 号：R446.62[医药卫生—诊断学]