基于微流控芯片的纤维蛋白原检测技术与实验研究  

Research on fibrinogen detection technology and experiment based on microfluidic chip

作　　者：李默 张思祥[1] 周围[1] 竭霞 王哲[1] 朱华波 LI Mo;ZHANG Sixiang;ZHOU Wei;JIE Xia;WANG Zhe;ZHU Huabo(School of Mechanical Engineering,Hebei University of Technology,Tianjin 300130,China)

机构地区：[1]河北工业大学机械学院,天津300130

出　　处：《传感器与微系统》2020年第4期13-15,19,共4页Transducer and Microsystem Technologies

摘　　要：针对纤维蛋白原(FIB)检测过程中由于FIB与凝血酶发生凝集反应引起浊度变化的特点,基于微流控技术,结合吸光度的光电检测方法,设计了一种专用微流控芯片。根据朗伯-比尔定律吸光度与待测液体浓度呈线性关系的性质,用特定波长光源照射经过相应处理的待测样本,最终通过吸光度标定换算出FIB的浓度。相比于传统检测方法,微流控芯片可以很大程度上对实验的变量进行简化和控制,实现了流体的可视化。通过COMSOL有限元法仿真,得出Y型芯片最佳角度为70°,分析血浆注入速度对凝血时间的影响,确定流量为30μL/s。仪器线性度良好,其方差系数为98. 32%。Aiming at the problem that the change of turbidity caused by blood coagulation during fibrinogen( FIB) detection based on microfluidic technology,combines the photoelectric detection method of absorbance,a special microfluidic chip is designed. According to the physical property of the Lambert Beer’s law,the absorbance is linearly related to the concentration of the liquid to be tested,concentration of fibrinogen can be converted by absorbance calibration from irradiation of samples to be measured with a specific wavelength light source.Compared with the traditional detection method,the microfluidic chip can simplify and control the experimental variables to a large extent. And it enables the visualization of fluids. Through COMSOL finite element simulation,the optimum angle of Y-chip is calculated as 70°. The effect of plasma injection rate on clotting time is analyzed and the flow rate is determined to be 30 μL/s. The instrument has good linearity with a variance coefficient of 98. 32 %.

关 键 词：纤维蛋白原浓度测定 微流控芯片 光电检测 有限元仿真 

分 类 号：TH773[机械工程—仪器科学与技术] TP212[机械工程—精密仪器及机械]