Molecular Detection of Sugarcane Ratoon Stunting Disease in Hainan Sugarcane-growing Areas  

作　　者：Shen Linbo Xiong Guoru Feng Xiaoyan Wang Wenzhi Feng Cuilian Zhao Tingting Wang Jungang Wu Nannan Zhang Shuzhen 

机构地区：[1]Sugarcane Research Center,Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources Utilization,Ministry of Agriculture and Rural Areas,Haikou 571101,China [2]College of Life Science,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Plant Diseases and Pests》2020年第1期6-9,共4页植物病虫害研究（英文版）

基　　金：Supported by National Natural Science Foundation of China(31771865);National Sugar Industry Technology System"Scientists for Prevention and Control of Sugarcane Virus Diseases"(CARS-170301)。

摘　　要：[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing areas.The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.

关 键 词：SUGARCANE SUGARCANE RATOON STUNTING disease(RSD) Molecular DETECTION DETECTION rate HAINAN 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]