高通量测序技术在转基因植物分子特征评价中的应用  

Genome Sequencing Application on Molecular Characterization Assessment of Transgenic Plant

作　　者：马硕 焦悦 王旭静[1] 翟勇 王志兴[1] MA Shuo;JIAO Yue;WANG Xujing;ZHAI Yong;WANG Zhixing(MARA Key Laboratory on Safety Assessment(Molecular)of Agri-GMO,Ministry of Agriculture and Rural Affairs,Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Development Center for Science and Technology,Ministry of Agriculture and Rural Affairs,Beijing 100122,China)

机构地区：[1]中国农业科学院生物技术研究所,农业农村部农业转基因生物安全评价(分子)重点实验室,北京100081 [2]农业农村部科技发展中心,北京100122

出　　处：《中国农业科技导报》2020年第5期6-14,共9页Journal of Agricultural Science and Technology

基　　金：农产品质量安全监管经费项目。

摘　　要：分子特征是筛选和鉴别转化体重要依据,也是转基因植物安全评价必须提供的内容。DNA测序技术的发展为精准解析转基因植物的分子特征提供了新的技术方案。综述了高通量测序技术在转基因植物分子特征中的应用及欧盟和加拿大对转基因植物安全评价中所提供测序数据的要求,并提出了我国转基因植物安全评价中采纳测序数据时的建议,为高通量测序技术在我国转基因生物安全评价中的应用提供理论支撑。Molecular characterization is an important basis for selecting and identifying transgenic event, and also the main content which must be provided for safety assessment of transgenic plant. The development of DNA sequencing technology provides a new strategy for accurately analyzing the molecular characterization of transgenic plants. This paper reviewed the development and technical principle of DNA sequencing technology and the application of high-throughput sequencing in the analysis of molecular characterization. In addition, it described the requirements of European Union and Canada for sequencing data provided for the safety assessment of transgenic plant. Based on above, adopt high-throughput sequencing data in the safety assessment of transgenic plant in China were proposed, which provided theoretical support for the application of high-throughput sequencing technology in the safety assessment of transgenic plant in China.

关 键 词：基因组测序 转基因植物 分子特征 安全评价 

分 类 号：Q78[生物学—分子生物学]