可沉微藻转化油脂潜力及PHB合成试验研究  

Lipids Conversion Potential and PHB Synthesis of Settleable Microalgae

作　　者：郝晓地[1] 靳景宜 罗玉琪[1] 曹达啓 HAO Xiao-di;JIN Jing-yi;LUO Yu-qi;CAO Da-qi(Sino-Dutch R&D Centre for Future Wastewater Treatment Technologies,Beijing Advanced Innovation Center of Future Urban Design,Beijing University of Civil Engineering and Architecture,Beijing100044,China)

机构地区：[1]北京建筑大学北京未来城市设计高精尖创新中心中-荷未来污水处理技术研发中心,北京100044

出　　处：《中国给水排水》2020年第7期1-6,共6页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51878022)。

摘　　要：可沉微藻在去除二级出水中氮、磷的同时,可以合成并积累多种具有高附加值的聚合物,如油脂、蛋白质、多糖、聚β-羟基丁酸酯(PHB)等。前期试验在"冲淘"压力下筛选出了沉淀率高达97%的可沉微藻,并通过一系列环境变化(通入CO2、实行光/暗循环等)刺激油脂聚积,最高可获得49.2%(细胞干质量,下同)的油脂含量。进一步强化试验显示,以增加光照强度与CO2通入量达到进一步提高可沉微藻油脂含量的做法事与愿违,反而出现油脂含量下降的现象。而可沉蓝藻具有相当的合成、积累PHB的能力,只要存在少许特异性碳源(乙酸盐)刺激,可沉蓝藻在不通入CO2的情况下即可轻松合成并积累含量高达30%左右的PHB。这种主要以无机碳源合成PHB的方法比以有机碳源合成PHB的活性污泥法更具吸引力。Beyond removing nitrogen and phosphorous from secondary effluent,the settleable microalgae can also synthesize and accumulate such high value-added polymers as lipids,proteins,polysaccharides,poly-β-hydroxybutyrate(PHB),etc.The previous experiments efficiently enriched settleable microalgae with the settleable rate of more than 97%by applying“washout”pressure,and accumulated the highest lipids content of 49.2%(dry cell weight)by changing environmental factors like CO2,light/dark shift,etc.According to the following experiment results,increasing the light intensity and CO2 intake would not further increase the lipids content of the settleable microalgae.Instead,the lipids content decreased.However,settleable cyanobacteria had a good ability to synthesize and accumulate PHB.As long as there was a tiny specific carbon source(acetate)stimulation,cyanobacteria could easily synthesize and accumulate PHB with the content of up to 30%without CO2 input.Clearly,synthesizing PHB mainly from inorganic carbon source was more attractive than that from organic carbon source by activated sludge.

关 键 词：可沉微藻 二级出水 聚合物 油脂 聚β-羟基丁酸酯(PHB) 蓝藻 

分 类 号：TU992[建筑科学—市政工程]