小麦有效分蘖数QTLs定位及其元分析  

Mapping and Meta-analysis of QTLs for the Productive Tiller Number in Wheat(Triticum aestivum)

作　　者：左煜昕 刘媛[1] 张沛沛 栗孟飞[1] 程宏波[1] 陈思瑾 幸华[1] 杨德龙[1] ZUO Yu-Xin;LIU Yuan;ZHANG Pei-Pei;LI Meng-Fei;CHENG Hong-Bo;CHEN Si-Jin;XING Hua;YANG De-Long(Gansu Provincial Key Lab ofAridland Crop Science/College of Life Science and Technology,GansuAgricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃省干旱生境作物学重点实验室/甘肃农业大学生命科学技术学院,兰州730070

出　　处：《农业生物技术学报》2020年第4期582-593,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31760385,31460348);甘肃省现代农业生产技术体系项目(GARS-01-04)。

摘　　要：有效分蘖数(productive tiller number, PTN)作为小麦(Triticum aestivum)产量形成重要因子之一,是由多基因控制的复杂数量性状,利用数量性状基因位点(quantitative trait loci, QTL)定位和元分析手段,发掘PTN真实的主效QTL及其紧密连锁的分子标记,是实现小麦分子遗传改良的重要途径。本研究利用1套小麦重组近交系(recombinant inbred lines, RIL)群体,设置不同水分条件,对PTN进行QTL定位,并整合已发表的QTL信息,进行元分析。结果发现,小麦RIL群体PTN表型变异广泛且存在超亲分离现象,易受水分环境影响,普遍表现出较低的遗传力。在不同水分环境条件下,共检测到22个控制小麦PTN的加性QTL,分布在除4A以外的20条染色体上,对表型变异的贡献率在5.86%~13.84%。通过整合来源于15个作图群体的97个控制PTN的QTL,建立了QTL一致性图谱。最终获得33个一致性QTL及其紧密连锁的候选分子标记,图距最小可达到1.94 cM。本研究结果为小麦有效分蘖数QTL精细定位以及分子标记辅助选择育种提供理论依据。Productive tiller number(PTN) is one of the important factors for the yield performance of wheat(Triticum aestivum) and a complex quantitative trait controlled by polygenes. In this case, it is critical to explore the main-effect quantitative trait loci(QTL) and its closely linked molecular markers by the strategies of QTL mapping and meta-analysis in molecular genetic improvement in wheat breeding. In this study, a set of recombinant inbred lines(RIL) of wheat under different water conditions was used to map QTLs for the PTN trait. These identified QTLs were further integrated with reported QTLs for meta-analysis. The results showed that phenotypic values of PTN in the RIL varied widely with significantly transgressive segregation which were sensitive to water stress and thus indicated a low heritability. Under different water conditions, a total of22 additive QTLs were identified and distributed on all chromosomes except chromosome 4 A, accounting for5.86%~13.84% of phenotypic variations. A total of 97 QTLs governing the PTN from 15 mapping populations were used to construct QTL consensus map in wheat. Consequently, 33 of meta QTLs and their closely linked molecular markers were available and confirmed. The minimum interval of meta QTL was 1.94 cM. The study would be useful to fine QTL mapping and molecular marker-assisted selection breeding for the productive tiller number in wheat.

关 键 词：小麦 有效分蘖数 QTL定位 元分析 

分 类 号：S512.1[农业科学—作物学] S332.4