四个绵羊品种BMPR-IB基因3’-UTR区多态性及其与胎产羔数的相关分析  

Polymorphism of 3’-UTR Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep(Ovis aries) Varieties

作　　者：孙渭博 张利平[1] 郎侠[2] 张筱艳[1] 靳继鹏 张紫奇 肖帆 雷志惠 SUN Wei-Bo;ZHANG Li-Ping;LANG Xia;ZHANG Xiao-Yan;JIN Ji-Peng;ZHANG Zi-Qi;XIAO Fan;LEI Zhi-Hui(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Institute of Animal and Pasture Science and Green Agriculture,Gansu Academy of Agricultural Science,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]甘肃省农业科学院畜草与绿色农业研究所,兰州730070

出　　处：《农业生物技术学报》2020年第4期702-710,共9页Journal of Agricultural Biotechnology

基　　金：公益性行业(农业)科研专项放牧牛羊营养均衡需要研究与示范(201303062);甘肃省农业科学院创新团队项目:动物遗传育种与草食畜生产体系;国家自然科学基金(31260547)。

摘　　要：为探究骨形态发生受体蛋白IB基因(bone morphogenetic protein receptor IB,BMPR-IB)非编码区多态性对绵羊(Ovis aries)繁殖性能的遗传效应,本实验以湖羊多羔母羊(Multiple Hu Sheep,HM)、蒙古羊单羔母羊(Singletons Mongolian Sheep,MS)和双羔母羊(Twins Mongolian Sheep,MT)、欧拉羊单羔母羊(Singletons Oula Sheep,ZS)和双羔母羊(Twins Oula Sheep,ZT)、澳洲白绵羊单羔母羊(Singletons Australian White Sheep,AS)为研究对象,利用DNA直接测序法检测BMPR-IB基因3'-非编码区(3'-untranslated region,3'-UTR)SNPs,并分析其与胎产羔数(后简称产羔数)之间的相关性。结果表明:在3'-UTR区1145~1500 bp检测到1339(G/A)和1354(A/G)两个突变位点,对3'-UTR区1354位点(对应BMPRIB基因mRNA序列NM_001009431.1中3019位点)进一步分析,表明在MT和ZT中检测到野生AA型、杂合突变AG型和纯合突变GG型,优势等位基因均为A;在MS、ZS及AS中检测到野生AA型和杂合突变AG型,优势等位基因均为A;在HM中检测到杂合突变AG型和纯合突变GG型,优势等位基因为G;经多态信息含量(polymorphism information content,PIC)分析可知:HM、MT、MS、ZS及AS处于低度多态(PIC<0.25);ZT处于中度多态(0.25<PIC<0.5);BMPR-IB基因3'-UTR区1354位点突变在产多羔、双羔群体与产单羔群体间存在显著差异(P<0.05)。AG、GG基因型对湖羊、产双羔的蒙古羊和欧拉羊实验群体的产羔数有显著影响,故该位点可作为产多羔及产双羔绵羊产羔性状的分子遗传标记位点。本研究为选育高繁殖力绵羊提供理论科学依据。In order to explore the genetic effects of the untranslated region polymorphism of bone morphogenetic protein receptor IB(BMPR-IB)gene on the reproductive performance of sheep(Ovis aries),Multiple Hu Sheep(HM),Singleton Mongolian Sheep(MS),Twins Mongolian Sheep(MT),Singletons Oula Sheep(ZS),Twins Oula Sheep(ZT)and Singletons Australian Sheep(AS)was selected in this study to detect SNPs in the 3'-UTR of BMPR-IB gene by DNA sequencing,the correlation between polymorphism loci and litter size was also studied.Two mutation sites of 1339(G/A)and 1354(A/G)were detected at 1145~1500 bp in the 3'-UTR region of BMPR-IB gene,and further analysis was performed on the 1354 locus(corresponding to the 3019 locus in the mRNA sequence of BMPR-IB gene NM_001009431.1).The results showed that wild genotype AA,heterozygous mutant genotype AG and homozygous mutant genotype GG were detected in MT and ZT,the dominant allele was A;wild genotype AA and heterozygous mutation genotype AG were detected in MS,ZS and AS,the dominant allele was A;heterozygous mutation genotype AG and homozygous mutation genotype GG were detected in HM,the dominant allele was G.The result of polymorphism information content(PIC)analysis showed that HM,MT,MS,ZS and AS were in low polymorphism(PIC<0.25);and ZT was in moderate polymorphism(0.25<PIC<0.5).There was a significant difference in the 1354 locus of 3'-UTR region of BMPR-IB gene among the multiple-lamb,twins-lamb and singletons-lamb populations(P<0.05).The genotype of AG and GG had significant effects on the fecundity in HM,MT and ZT.These results suggested that the 1354 locus in the 3'-UTR region of BMPR-IB gene might be regarded as a molecular genetic marker locus for multiple-lamb and twins-lamb in sheep.This study provides a theoretical scientific basis for breeding sheep with high fecundity.

关 键 词：绵羊 骨形态发生蛋白受体-IB基因(BMPR-IB) 3'-UTR 多态性 

分 类 号：S813.3[农业科学—畜牧学]