布鲁氏菌血清抗体量子点荧光微球检测试纸条的研制  

Establishment of a quantum dots fluorescent microspheres based immunochromatographic strip for detection of the antibodies against brucellosis

作　　者：孔玉方 王慧煜[1] 梅琳[1] 韩雪清[1] KONG Yu-fang;WANG Hui-yu;MEI Lin;HAN Xue-qing(Chinese Academy of Inspection and Quarantine Sciences,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《中国兽医科学》2020年第5期545-549,共5页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2016YFD0501106);中国检验检疫科学研究院基本科研业务费项目(2018JK012)。

摘　　要：为研制一种现场快速筛查布鲁氏菌病的免疫层析试纸条,本研究以量子点荧光微球作为示踪物,共价偶联布鲁氏菌外膜蛋白OMP22,并将布鲁氏菌外膜蛋白OMP28和抗OMP22的单克隆抗体喷涂于硝酸纤维素膜上分别作为检测线和质控线,组装试纸条。结果显示,量子点荧光微球试纸条检测阳性血清的最大稀释度为1∶512,检测布鲁氏菌病阳性血清的敏感性为99%,特异性为98%,与虎红平板凝集试验(RBPT)的符合率为99%。且与结核病、蓝舌病、病毒性腹泻、口蹄疫及小反刍兽疫血清无交叉反应。上述结果表明量子点荧光微球免疫层析试纸条检测速度快、灵敏度高、特异性强且成本低,适用于布鲁氏菌病的现场筛查。In order to establish an immunochromatographic test strip for rapid screening of brucellosis in the field,the test strips was successfully prepared by the quantum dot fluorescent microspheres as tracers,which was covalently coupling Brucella outer membrane protein OMP22.Then outer membrane protein OMP28 and monoclonal antibodies of OMP22 of the genus Brucella were sprayed on the nitrocellulose membrane as test lines and quality control lines,respectively.The results demonstrated that the maximum dilution of the positive serum of the quantum dot fluorescent microsphere test strip was 1∶512.The sensitivity of detecting positive serum was 99%and the specificity was 98%,and the coincidence rate with the tiger red plate agglutination test(RBPT)was 99%,in addition,there was no cross reaction with the sera of other related disease.The above results indicate that the quantum dot fluorescent microsphere immunochromatographic test strip has potential promising applications for on-site screening of brucellosis due to its high detection speed,high sensitivity,high specificity and low cost.

关 键 词：布鲁氏菌 量子点荧光微球 外膜蛋白 

分 类 号：S852.614[农业科学—基础兽医学]