REV感染对SPF雏鸡胸腺细胞增殖和细胞周期及周期素D1的影响  

Effect of REV infection on proliferation,cell cycle and Cyclin D1 of thymocytes in SPF chickens

作　　者：吴乐 高海婷 刘超男[1] 高雪丽[1] 张淑君 周放 吕晓萍[1] 边洋 郑世民[1] WU Le;GAO Hai-ting;LIU Chao-nan;GAO Xue-li;ZHANG Shu-jun;ZHOU Fang;LU Xiao-ping;BIAN Yang;ZHENG Shi-min(College of Veterinary Medicine,Northeast Agricultural University,Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine,Harbin 150030,China)

机构地区：[1]东北农业大学动物医学学院,黑龙江省实验动物与比较医学重点实验室,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2020年第5期643-649,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31472169)。

摘　　要：为了探索禽网状内皮组织增生病病毒(REV)感染SPF雏鸡后,对其胸腺细胞增殖、细胞周期分布以及Cyclin D1的影响,将66只1日龄SPF雏鸡随机分为REV感染组和对照组,使用Cell counting Kit-8、流式细胞术、Western-blot、荧光定量PCR等方法对雏鸡胸腺细胞增殖、细胞周期及Cyclin D1 mRNA和蛋白的动态变化进行检测。结果发现,REV感染SPF雏鸡后,其胸腺细胞的增殖能力于第14、21、28天明显低于对照雏鸡;G0/G1期细胞数于第14天较对照雏鸡明显(P<0.05)升高,S、G2/M期细胞数量减少,第28天时相反;Cyclin D1 mRNA表达于第14天极显著(P<0.01)低于对照雏鸡,第35天时极显著高于(P<0.01)对照雏鸡;Cyclin D1蛋白量显著(P<0.05)或极显著(P<0.01)低于对照雏鸡。结果表明,感染REV后,雏鸡免疫机能下降可能与胸腺细胞增殖能力下降、细胞周期素D1 mRNA转录及蛋白质水平的异常表达相关。To explore the effects of reticuloendotheliosis virus(REV)infection on thymocyte proliferation,cell cycle and Cyclin D1 expression in SPF chickens.Sixty-six 1-day-old SPF chickens were randomly divided into REV-infected group and control group.Cell counting Kit-8,flow cytometry,Western-blot,real-time PCR and other methods were used to analyze the dynamic changes of thymocyte proliferation,cell cycle distribution and Cyclin D1 expression in chickens.The results showed that,after SPF chickens infected with REV,the proliferation in thymocytes significantly lower than controls on days 14,21,28,the number of cells in G0/G1 phase was significantly higher(P<0.05)than that in control group on day 14,and the number of cells in S and G2/M phases was decreased,the data on day 28 is the opposite to day 14.Cyclin D1 mRNA expression was extremely significant(P<0.01)lower than control chicks on day 14 and significantly higher than(P<0.01)control on day 35,in the REV-infected chickens,the content of Cyclin D1 protein was significant(P<0.05)or extremely significant(P<0.01)lower than in the control group.These results suggest that REV infection can reduce the immune function of chickens,which may be related to the decreased proliferation of thymocytes and the abnormal expression levels of Cyclin D1 in SPF chickens.

关 键 词：SPF雏鸡 禽网状内皮组织增生病病毒 胸腺细胞 细胞周期 细胞周期素D1 

分 类 号：S852.43[农业科学—基础兽医学]