Determination of Bisoprolol Fumarate by Fluorescence Quenching Method  

作　　者：Yu LIN Xiaomei LAN 

机构地区：[1]Guangxi University of Chinese Medicine, Nanning 530001, China

出　　处：《Medicinal Plant》2020年第2期30-32,37,共4页药用植物：英文版

基　　金：Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2017(2017KY0284);Project of Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GXZYKF2019-7);Program of Key Laboratory for Extraction and Purification and Quality Analysis of TCM in 2017(J1700208);Project of Guangxi University of Chinese Medicine(YB14004);Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine([2013]No.20).

摘　　要：[Objectives]To establish a new method for indirect determination of bisoprolol fumarate based on fluorescence quenching technology.[Methods]In ammonia water and ammonium chloride buffer solution at pH=9.2,whenλexcitation=277 nm andλemission=596 nm,with the increase of CCu2+,the fluorescence signal intensity of bisoprolol fumarate weakened,and the difference between the fluorescence intensity of bisoprolol fumarate itself and the fluorescence intensity of the test solution after the quencher Cu2+was added(ΔF)and Cbisoprolol fumarate showed a good linear relationship.[Results]In the range of 15.39-76.93μg/mL,ΔF=146.7 Cbisoprolol fumarate+482.1,r=0.9988,and the detection limit is 0.1391μg/mL.[Conclusions]The fluorescence quenching method has been applied to the determination of actual samples with a recovery rate of 99.9%and an RSD of 2.7%.The results are satisfactory.

关 键 词：BISOPROLOL FUMARATE COMPLEXING FLUORESCENCE QUENCHING method Content DETERMINATION 

分 类 号：TG1[金属学及工艺—金属学]