Optimization of Extraction of Total Flavonoids from HERBA BLUMEAE RIPARIAE Using Response Surface Methodology  

作　　者：Hongna SU Yining ZHANG Xuexue LI Xuelian ZHAO Yisong LI Wenbing LI Yuan LIU 

机构地区：[1]College of Pharmacy, Southwest Minzu University, Chengdu 610041, China [2]Institute of Qinghai-Tibetan Plateau Research, Southwest Minzu University, Chengdu 610225, China [3]Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory, Chengdu 610225, China [4]Ethnic Medicine Institute, Southwest Minzu University, Chengdu 610041, China

出　　处：《Medicinal Plant》2020年第2期73-76,共4页药用植物：英文版

基　　金：National Key Research and Development Program of China(2018YFC1708005);Applied Basic Research Project of Sichuan Province 2020(20YYJC3299);Fundamental Research Funds for the Central Universities(2020NGD01).

摘　　要：[Objectives]This study aimed to optimize the ultrasonic-assisted extraction process of total flavonoids from a Zhuang medicine HERBA BLUMEAE RIPARIAE using response surface methodology.[Methods]Using rutin as a reference substance,a standard curve was drawn at a wavelength of 292 nm.Content determination was performed through absorbance under different conditions.The effects of ultrasonic time,ethanol volume fraction and liquid-to-solid ratio on the yield of total flavonoids from HERBA BLUMEAE RIPARIAE were investigated by single-factor tests.By using Box-Behnken test,the ultrasonic-assisted extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was optimized.[Results]The optimal extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was as follows:ultrasonic time of 30 min,ethanol volume fraction of 50%and liquid-to-solid ratio of 40∶1(mL/g).Under the optimal extraction conditions,the average yield of total flavonoids from HERBA BLUMEAE RIPARIAE was 85.47 mg/g.[Conclusions]The optimized extraction process was convenient and feasible.This study will provide a reference for the extraction of total flavonoids from HERBA BLUMEAE RIPARIAE,and provide certain guidance and basis for further development and utilization of the plan resource of Blumea riparia(Bl.)DC.

关 键 词：Zhuang MEDICINE HERBA BLUMEAE RIPARIAE TOTAL FLAVONOIDS RESPONSE SURFACE optimization EXTRACTION process 

分 类 号：R28[医药卫生—中药学]