冠心平含药血清对LPS诱导的RAW264.7巨噬细胞极化的影响  

Effects of Guanxinping-Containing Serum on RAW 264.7 Macrophages Polarization Induced by LPS

作　　者：周冠进 孙玉婷 周李煜 陈韦凯 掌琳惠 胡文祺 刘福明[1] 袁冬平[2] ZHOU Guan-jin;SUN Yu-ting;ZHOU Li-yu;CHEN Wei-kai;ZHANG Lin-hui;HU Wen-qi;LIU Fu-ming;YUAN Dong-ping(Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China)

机构地区：[1]南京中医药大学附属医院/江苏省中医院,江苏南京210029 [2]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2020年第3期376-379,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药科技发展计划重点项目(ZD201906);江苏省“六大人才高峰”创新人才团队项目(TD-SWYY-069);江苏省中医院创新发展基金(Y2019CX20)。

摘　　要：目的探讨冠心平(GXP)含药血清对LPS诱导的RAW264.7巨噬细胞极化的调控作用。方法RAW264.7巨噬细胞分为对照组、模型组、GXP低、中、高剂量组,100 ng/mL LPS刺激12 h诱导M1型极化模型,不同浓度GXP含药血清进行干预。ELISA法检测巨噬细胞上清肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)含量;流式细胞术检测M1、M2型巨噬细胞特征性表面标记分子CD86、CD206表达水平;qPCR法检测巨噬细胞CD86、CD206 mRNA表达情况。结果冠心平高剂量显著减少巨噬细胞TNF-α分泌(P<0.01),各剂量减少TGF-β1分泌(P<0.01);冠心平低、高剂量显著降低M1型巨噬细胞特征性分子CD86表达(P<0.05),显著升高M2型巨噬细胞特征性分子CD206表达(P<0.01);同时冠心平CD206 mRNA表达显著增加(P<0.05)。结论冠心平含药血清能够抑制LPS诱导的巨噬细胞M1型极化而促进M2型极化,这或许是冠心平治疗动脉粥样硬化的潜在机制之一。OBJECTIVE To investigate the effects of Guanxinping(GXP)on RAW264.7 macrophage polarization induced by LPS.METHODS Macrophages were divided into control group,model group and low-,mid-,high-dose of GXP groups.Cells were starved for 24 hours before intervention with drug-containing serum of rats.The characteristic molecules of macrophage polarization in cell supernatant,including tumor necrosis factorα(TNF-α)and transforming growth factorβ(TGF-β)were detected by ELISA;the expression levels of M1 and M2 macrophage surface molecules CD86 and CD206 were detected by flow cytometry;the expression of mRNA of CD86 and CD206 were detected by qPCR.RESULTS Compared to the model group,high-dose of GXP decreased the secretion of TNF-α(P<0.01);low-,mid-,high-dose of GXP decreased the secretion of TGF-β1(P<0.01)in macrophage supernatant.Low-and high-dose of GXP decreased the expressions of CD86(P<0.05)and increased the expression of CD206(P<0.01).There were no significant difference in the mRNA expression of CD86 between the GXP groups and model group,but GXP increased the mRNA expression of CD206.CONCLUSION GXP can inhibit the polarization of macrophages towards M2,which may be one of the mechanisms of GXP in the treatment of atherosclerosis.

关 键 词：冠心平 动脉粥样硬化 巨噬细胞 极化 

分 类 号：R285.5[医药卫生—中药学]