一种模块化三维灌注培养系统的研制  

Development of modular three-dimensional perfusion cellculture system

作　　者：刘鹏[1,2] 向俊西 杨丽斐[2] 史爱华 刘爽 吕毅 LIU Peng;XIANG Jun-xi;YANG Li-fei;SHI Ai-hua;LIU Shuang;LYU Yi(Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院肝胆外科,西安710061 [2]精准外科与再生医学国家地方联合工程研究中心,西安710061

出　　处：《医疗卫生装备》2020年第5期47-50,92,共5页Chinese Medical Equipment Journal

基　　金：国家自然科学基金(81501608)。

摘　　要：目的:研制一种模块化三维灌注培养系统,解决目前培养系统结构复杂、难消毒、易污染的问题。方法:该系统主要由生物反应器模块、氧合模块、动力模块以及换液模块构成,各模块壳体通过3D打印制作。其中生物反应器模块主体为80 ml蓝口瓶,装载培养基及细胞支架复合物。氧合模块装载膜肺、空气过滤器、气泡过滤器等,为培养系统的核心组件。动力模块由蠕动泵和空气泵组成,为培养基及气体提供动力。换液模块中装有深紫外LED灯,可进行消毒和更换培养基。结果:通过该系统进行大鼠脾脏脱细胞支架培养,细胞与支架黏附紧密、生长状态良好,且未发生污染。结论:该系统具有结构合理、易消毒、污染少、制作成本低、稳定性高、易于扩展且操作简单的优点,可很方便地进行基于脱细胞支架的三维灌注培养,值得推广应用。Objective To develop a modular three-dimensional perfusion culture system to eliminate the shortcomings of the existing system in complicated structure,difficult disinfection,vulnerability to pollution and etc.Methods The system was composed of several modules for bioreactor,oxygenation,power and liquid changing,and all the shells were made by 3D printing.The bioreactor module had an 80 ml blue bottle as its main body,which was loaded with culture medium and cell scaffold complex.The oxygenation module was equipped with membrane lung,gas filter,bubble filter,etc.,which was the core component of the culture system.The power module consisted of a peristaltic pump and an air pump to provide power for the medium and gas.The liquid changing module included a deep UV LED lamp,which could be used to disinfect and change the medium.Results The system was used to culture the acellular scaffold of rat spleen,and the cells adhered to the scaffold tightly and grew well without pollution.Conclusion The perfusion culture system developed has the advantages of reasonable structure,easy disinfection,few pollution,low production cost,high stability,easy expansion and simple operation,and thus is worthy promoting for three-dimensional perfusion culture of decellularization scaffold.

关 键 词：三维灌注培养 模块化 3D打印 脱细胞支架 细胞培养 

分 类 号：R318[医药卫生—生物医学工程] Q813.1[医药卫生—基础医学]