UPLC-MS/MS法同时测定木蝴蝶中10个有效成分  

Simultaneous determination of ten components in Oroxylum indicum by UPLC-MS/MS

作　　者：魏欣[1] 杨金霞 孙雅晴 张炜[2] 剧杰茜 祁金龙[2] WEI Xin;YANG Jin-xia;SUN Ya-qing;ZHANG Wei;JU Jie-xi;QI Jin-long(Department of Pharmacy,Hebei People’s Hospital,Shijiazhuang 050051,China;Department of Pharmacology,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北省人民医院药学部,河北石家庄050051 [2]河北医科大学药理学教研室,河北石家庄050017

出　　处：《中草药》2020年第8期2221-2225,共5页Chinese Traditional and Herbal Drugs

基　　金：河北省卫计委课题(20170287);河北省高等学校科学技术研究重点项目(ZD2019054);河北省自然科学基金面上项目(H2019206219)。

摘　　要：目的建立UPLC-MS/MS方法同时测定木蝴蝶中木蝴蝶苷A、木蝴蝶苷B、黄芩苷、黄芩素、白杨素、高车前素、野黄芩素、金丝桃苷、芹菜素、槲皮素10种主要成分。方法采用负离子多反应监测(MRM)模式,ESI离子源,Kinetex C18色谱柱(100 mm×3.0 mm,2.6μm)进行分离,流动相为乙腈-甲醇-0.5%甲酸水,梯度洗脱,分析时间为7 min。结果所测10种主要成分在测定浓度范围内线性关系良好,相关系数均大于0.9966;精密度、重复性和稳定性良好;平均加样回收率为94.0%~104.5%,RSD≤3.21%。结论首次建立可同时测定木蝴蝶中10种主要成分的UPLC-MS/MS方法,该法操作简便,灵敏度高,专属性好,分析速度快,可用于中药材木蝴蝶的质量控制。Objective To develop and validate an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous determination of ten component(baicalin-7-glucoside,oroxin BCAS,baicalin,baicalein,chrysin,hispidulin,scutellarein,hyperoside,apigenin and quercetin)in Oroxylum indicum.Methods The ten components were separated within 7.0 min on a Kinetex C18 column(100 mm×3.0 mm,2μm)using a mobile phase consisted of methanol,acetonitrile and 0.1%formic acid water solution with gradient elution.Results All of the analytes showed good linearity(r≥0.9966)in the tested ranges.The precision,repeatability and stability of the method were good for the ten components.The average recoveries were in the range of 94.0%-104.5%with relative standard deviations(RSD)≤3.21%.Conclusion A new rapid and reliable UPLC-MS/MS method was developed and validated to evaluate the quality of O.indicum for the first time.

关 键 词：木蝴蝶 UPLC法 木蝴蝶苷A 木蝴蝶苷B 黄芩苷 黄芩素 白杨素 高车前素 野黄芩素 金丝桃苷 芹菜素 槲皮素 

分 类 号：R286.2[医药卫生—中药学]