利用SSR标记对水稻7组衍生品种的遗传多样性分析  

Analysis on genetic diversity of seven groups of rice-derived varieties based on SSR marker

作　　者：马菊 赵锦祥 林郑希 谢永波 陈志伟[1] 毛大梅[1] 周彬 官华忠[1] MA Ju;ZHAO Jinxiang;LIN Zhengxi;XIE Yongbo;CHEN Zhiwei;MAO Damei;ZHOU Bin;GUAN Huazhong(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops/Fujian Provincial Key Laboratory of Crop Breeding by Design,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2020年第3期289-294,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家重点研究计划(2017YFD0100100);福建省科技厅区域发展项目(2018N3011);福建省科技厅引导项目(2018N006)资助;福建农林大学科研发展基金(KFA17166A).

摘　　要：采用53对SSR引物对水稻7组衍生品种及其亲本进行遗传多样性和标记鉴定效率分析.结果表明,53对SSR引物中47对具有多态性,占88.68%;共检测到135个等位位点,每个标记平均2.8720个等位位点,变幅为2~5;多态性信息含量(PIC)平均值为0.4821,变幅为0.2142~0.7891,其中14对引物具有高的PIC值(>0.6).聚类分析表明,7组供试衍生品种能按来源被准确分类.比较24对引物(NY/T 1433-2007)和48对引物(NY/T 1433-2014)的鉴定效率发现,标记数增加能提高鉴定准确性.The genetic diversity and identification efficiency of 7 groups of cultivars which were derived from rice, and their parents were evaluated by SSR markers. The results showed that 47 SSR primers were polymorphic, accounting for 88.68% of 53 primers. A total of 135 alleles were detected,averaged at 2.872 0 per primer and ranged from 2 to 5. The average of polymorphism information content was 0.482 1, varying between 0.214 2 and 0.789 1. Among them, 14 primers had higher polymorphism information content(>0.6). Cluster analysis revealed that 7 groups of the derived varieties were all accurately classified by source. By comparing the identification efficiencies of 24 primers from NY/T 1433-2007 and 48 primers from NY/T 1433-2014, it can be concluded that the more markers, the higher accuracy of the identification would be.

关 键 词：水稻 SSR标记 遗传多样性 

分 类 号：S511.01[农业科学—作物学] S311