光滑黄皮茎中香豆素类和生物碱类化学成分研究  

Study on coumarins and alkaloids from stems of Clausena lenis

作　　者：鄢贵 乔泽华 吴英菊 周俊宇 张栩铮 刘艳萍[2,3] 付艳辉[1,2,3] YAN Gui;QIAO Ze-hua;WU Ying-ju;ZHOU Jun-yu;ZHANG Yu-zheng;LIU Yan-ping;FU Yan-hui(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University,Haikou 571158,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]海南师范大学南药资源产业化关键技术研究海南省工程研究中心,海南海口571158 [3]海南师范大学热带药用资源化学教育部重点实验室,海南海口571158

出　　处：《中草药》2020年第7期1825-1830,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31660097);国家自然科学基金资助项目(21662011);国家自然科学基金资助项目(21967008);海南省重点研发计划项目(ZDYF2019049);海口市重点研发计划项目(2017050);教育部创新团队项目(IRT-16R19)。

摘　　要：目的研究芸香科黄皮属植物光滑黄皮Clausena lenis茎中的化学成分。方法综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离和纯化,根据化合物的理化性质及其波谱数据,并通过与文献中报道的波谱数据进行对照,鉴定化合物的化学结构。结果从光滑黄皮茎的90%乙醇提取物中分离得到了18个化合物,分别鉴定为6,8-diprenylumbelliferone(1)、byakangelicin(2)、tert-O-methylheraclenol(3)、pabularinone(4)、异栓翅芹烯醇(5)、独活属醇(6)、欧前胡素(7)、前胡醇(8)、花椒树皮素甲(9)、异紫花前胡内酯(10)、茵芋碱(11)、香草木宁(12)、N-metilatanina(13)、clausine Z(14)、九里香碱(15)、2-methoxy-1-(3-methyl-buten-1-yl)-9H-carbazole-3-carbaldehyde(16)、claulansine I(17)和clausehainanine A(18)。其中化合物1~10为香豆素类化合物,11~18为生物碱类化合物。结论所有化合物均为首次从光滑黄皮中分离得到,其中化合物2、3和8为首次从黄皮属植物中分离得到。Objective To study the coumarins and alkaloids from the stems of Clausena lenis.Methods The chemical constituents from the stems of C.lenis were separated and purified by silica gel,ODS,Sephadex LH-20 gel column chromatographies and preparative HPLC.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as the comparisons with the data reported in literatures.Results A total of 18 compounds were isolated from the 90%ethanol extract of the stems of C.lenis,which were identified as 6,8-diprenylumbelliferone(1),byakangelicin(2),tert-O-methylheraclenol(3),pabularinone(4),isogosferol(5),heraclenol(6),imperatorin(7),decursinol(8),xanthyletin(9),marmesin(10),skimmianine(11),kokusaginine(12),N-metilatanina(13),clausine Z(14),murrayanine(15),2-methoxy-1-(3-methyl-buten-1-yl)-9H-carbazole-3-carbaldehyde(16),claulansine I(17)and clausehainanine A(18).Among them,compounds 1—10 were coumarins and 11—18 were alkaloids.Conclusion All compounds are isolated from C.lenis for the first time,compounds 2,3 and 8 are separated from the genus Clausena for the first time.

关 键 词：光滑黄皮 香豆素 生物碱 异栓翅芹烯醇 独活属醇 欧前胡素 前胡醇 花椒树皮素甲 

分 类 号：R284.1[医药卫生—中药学]