白芍总苷对大鼠化学性肝损伤与肝阴虚证结合模型的影响和机制研究  

Effect and mechanism of total glucosides of Paeoniae Radix Alba on combined model of chemical liver injury and liver yin deficiency syndrome in rats

作　　者：贾岚 王蕾蕾 孟靓 华姞安 魏益谦 孟智睿 高学敏[1] 王景霞[1] JIA Lan;WANG Lei-lei;MENG Liang;HUA Ji-an;WEI Yi-qian;MENG Zhi-rui;GAO Xue-min;WANG Jing-xia(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《中草药》2020年第7期1885-1892,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81673617);国家中医药管理局中医药行业科研专项(201507004)。

摘　　要：目的研究白芍总苷对大鼠化学性肝损伤肝阴虚证候模型的影响和作用机制。方法SPF级雄性SD大鼠适应性喂养后,随机分为对照组、模型组、一贯煎组和白芍总苷组。除对照组外,其余各组大鼠ip 20%CCl4橄榄油溶液,后期ig给予附子、干姜、肉桂温热中药复方制备化学性肝损伤肝阴虚证模型。一贯煎组、白芍总苷组造模同时给药,1次/d,连续6周。6周后测定大鼠体质量、肛温,观察其一般状态;测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、6-酮-前列腺素F1α(6-keto-PGF1α)、血浆血栓素B2(TXB2)、层黏连蛋白(LN)、透明质酸(HA)水平及肝组织白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)水平;检测肝脏磷脂酰肌醇激酶(PI3k)mRNA、蛋白激酶B(Akt)mRNA和蛋白表达水平,并取肝脏进行HE病理切片检查。结果与模型组比较,白芍总苷组大鼠体征评分有明显改善(P<0.05),体质量显著升高(P<0.05),血清ALT、AST水平显著降低(P<0.01、0.001),cAMP/cGMP显著升高(P<0.01),LN、HA水平均显著降低(P<0.05、0.001),肝组织TNF-α、IL-6水平显著降低(P<0.001),Akt蛋白表达水平显著降低(P<0.05),PI3K、Akt mRNA表达水平显著降低(P<0.05、0.001),同时肝脏病理损伤有所改善。结论白芍总苷具有保护化学性肝损伤肝阴虚证的功效,可能是白芍养肝阴的物质基础,其作用机制可能与抗炎、减轻微循环障碍、通过抑制PI3K-Akt通路来调节细胞因子IL-6和IL-10平衡有关。Objective Based on the establishment of experimental model of liver yin deficiency syndrome,the effects of total glucosides of Paeoniae Radix Alba on the syndrome model of liver yin deficiency syndrome in rats with chemical liver injury were studied.Methods SPF male Sprague-Dawley rats weighing(200±20)g,after adaptive feeding,were randomly divided into control group,model group,Yi Guan Jian group,and total glycosides of Paeoniae Radix Alba group.Except the control group,the rats in the other groups were intraperitoneally injected with 20%CCl4 olive oil solution,and then ig treated with Aconiti Lateralis Radix Praeparata,Zingiberis Rhizoma and Cinnamomi Cortex to establish liver yin deficiency syndrome model.The Yi Guan Jian group and the total glucoside of Paeoniae Radix Alba group were administered at the same time,once a day for 6 weeks.After 6 weeks,the body weight and anal temperature of the rats were measured,and the general state was observed.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),6-keto-prostaglandin F1α(6-keto-PGF1α),plasma thrombosis content of B2(TXB2),laminin(LN)and hyaluronic acid(HA)were measured,and the activity of liver tissue interleukin-6(IL-6),IL-10,and tumor necrosis factor(TNF-α)were measured.The expression levels of phosphatidylinositol kinase(PI3k)mRNA,protein kinase B(Akt)mRNA and protein were determined and livers were taken for pathological examination of HE.Results Compared with the model group,the scores of the total glucosides of Paeoniae Radix Alba group were significantly increased(P<0.05),the body weight was significantly increased(P<0.05),and the serum ALT and AST levels were significantly decreased(P<0.01,0.001).The cAMP/cGMP ratio was increased significantly(P<0.01).The LN and HA of the liver tissue of the total glycosides group were significantly decreased(P<0.05,0.001).The content of TNF-αand IL-6 was decreased significantly(P<0.001),the expression level of Akt protein wa

关 键 词：白芍总苷 化学性肝损伤 肝阴虚证 丙氨酸转氨酶 天冬氨酸转氨酶 环磷酸腺苷 环磷酸鸟苷 6-酮-前列腺素F1Α 血浆血栓素B2 层黏连蛋白 透明质酸 白细胞介素-6 肿瘤坏死因子-α PI3K-Akt 

分 类 号：R285.5[医药卫生—中药学]