pH调控下基于分子内电荷转移(ICT)机理的硫化氢荧光探针  

An Intramolecular Charge Transfer(ICT)-Based Fluorescent Probe of Hydrogen Sulphide under pH Control Strategy

作　　者：朱继华[1,2] 张浩[1] 刘敏 刘旌江 廖原 权正军 王喜存[1] Zhu Jihua;Zhang Hao;Liu Min;Liu Jingjiang;Liao Yuan;Quan Zhengjun;Wang Xicun(College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070.;College of Chemistry and Chemical Engineering,Longdong University,Qingyang,Gansu 745000)

机构地区：[1]西北师范大学化学化工学院,兰州730070 [2]陇东学院化学化学工院,甘肃庆阳745000

出　　处：《有机化学》2020年第4期1043-1049,共7页Chinese Journal of Organic Chemistry

基　　金：陇原青年创新创业人才工程(No.2019-39);甘肃省高等学校科研(No.2018A-004)资助项目.

摘　　要：硫化氢(H2S)和pH在环境和细胞内的许多过程中起着至关重要的作用.为了更好地研究其功能,需要一种快速、灵敏地检测H2S和pH的荧光探针.设计合成了以萘二酰亚胺为荧光基团,叠氮为识别位点,基于分子内电荷转移(ICT)机制的反应型荧光探针L.该探针L在较宽的pH范围(4~11)内稳定性好,对H2S的选择性高,响应速度快(3 min),检测限(1.18μmol L^-1)较低,在0~20μmol L^-1的范围内,荧光强度与H2S浓度呈良好的线性关系(R^2=0.99823).此外,当体系中存在30倍的H2S时,在2≤pH≤6.5的范围内,L对pH变化显示线性关系(R^2=0.98764),可用作pH探针.该探针在检测H2S和30倍H2S存在条件下研究细胞微环境pH变化的方法,在分析检测和病理分析等方面具有潜在的应用前景.Hydrogen sulfide(H2S)and pH play a most important role in vivo and environment.A fluorescent probe for fast and sensitive detection of H2S and pH are of vital importance for research their functions.A naphthalimide-based fluorescent probe L has been designed and synthesized based on intramolecular charge transfer(ICT)mechanism by utilizing azide as recognition site.The probe L has demonstrated good stability in the pH range of 4~11.It showed satisfactory sensitivity and rapidly response(less than 3 min)to H2S.An excellent linear relationship(R^2=0.99823)displayed in the range of H2S concentrations from 0μmol L^-1 to 20μmol L^-1 and the detection limit was 1.18μmol L^-1.Furthermore,in the present 30 equiv.H2S,it was successfully applied to detection of pH with good linear relationship(R^2=0.98764)in the pH range of 2~6.5.This methods provide a reference for detection of H2S and pH value in the presence of 30 equiv.H2S,which have potential application prospects in analytical detection and pathological analysis.

关 键 词：硫化氢 PH 荧光探针 理论计算 ICT机制 

分 类 号：O657.3[理学—分析化学] R313[理学—化学]