RP-HPLC法同时测定翅碱蓬中四种黄酮化合物的含量  

Simultaneous determination of four flavonoids in Suaeda Heteroptera by RP-HPLC

作　　者：郭丽霞 宁寰宇 曹苇 李慧 孔亮 谭成玉 GUO Li-xia;NING Huan-yu;CAO Wei;LI Hui;KONG Liang;TAN Cheng-yu(College of Marine Science and Environment,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023

出　　处：《海洋科学》2020年第4期111-115,共5页Marine Sciences

基　　金：辽宁省自然科学基金重点项目(20170540102),盘锦红海滩湿地退化与生态修复项目(PHL-XZ-2017013002)。

摘　　要：建立RP-HPLC(反相高效液相色谱法)同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),检测波长为361nm,柱温为30℃,流动相为甲醇-0.4%冰乙酸(55︰45),流速1.0mL/min,四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001~0.01 mg/mL范围内呈现良好线性关系,相关系数R2在0.9990~0.9996,加标回收率(n=3)范围为82.39%~99.03%。该方法简单、快速、高效,可以用于翅碱蓬中四种黄酮化合物的含量分析。In this study,we established a reversed-phase high-performance liquid chromatography(RP-HPLC)method for the simultaneous determination of four flavonoids-rutin,quercetin 3-β-D-glucoside,narcissoside,and quercetin-in Suaeda Heteroptera.The separation was achieved using a Kromasil C18 column(4.6 mm×250 mm,5μm)at 30℃,with methanol-0.4%glacial acetic acid(55︰45)as the mobile phase at a flow rate of 1.0 mL.min–1 and a detection wavelength of 361 nm.The correlation coefficients ranged between 0.9990 and 0.9996 with calibration curves in the range of 0.001–0.01 mg·mL–1 for the four flavones.The average recoveries(n=9)obtained were 82.39%–99.03%.This method is simple,rapid,and efficient,and can be used for the simultaneous determination of rutin,quercetin 3-β-D glucoside,narcissoside,and quercetin in Suaeda Heteroptera samples.

关 键 词：翅碱蓬 反相高效液相色谱 芸香苷 槲皮素3-β-D-葡萄糖苷 水仙苷 槲皮素 

分 类 号：O629.9[理学—有机化学]