利用MALDI-TOF MS检测鲍曼不动杆菌对替加环素耐药性的研究  

Detection of tigecycline resistance of Acinetobacter baumannii by MALDI-TOF MS

作　　者：李蓉蓉 方亚平[2] 李亚娟 沈继录[1,3] Li Rongrong;FangYaping;Li Yajuan(Dept of Laborators Medicine,The First Affiliated Hospital qf Anhui Medical University,Hefei 230032;Dept of Laborators Medicine,The Second Affiliated Hospital of Anhui Medical Unwersity,Hefei 230601)

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230032 [2]安徽医科大学第二附属医院检验科,合肥230601 [3]安徽医科大学第四附属医院检验科,合肥230011

出　　处：《安徽医科大学学报》2020年第4期540-544,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年科学基金(编号:31600598)。

摘　　要：目的评价基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)技术对鲍曼不动杆菌的鉴定效果,同源性分析能力及自建库效果。方法利用MALDI-TOF MS技术鉴定107株鲍曼不动杆菌,再进行药物敏感性分析,筛选出替加环素耐药和敏感的鲍曼不动杆菌,利用筛选出的鲍曼不动杆菌建立替加环素耐药和敏感鲍曼不动杆菌数据库,最后进行自建库验证。结果 MALDI-TOF MS将107株细菌鉴定为103株鲍曼不动杆菌、3株琼氏不动杆菌和1株医院不动杆菌,利用K-B法和微量肉汤稀释法检测103株鲍曼不动杆菌对替加环素敏感性,得到37株替加环素耐药和39株替加环素敏感的鲍曼不动杆菌,两种方法的一致率为68. 82%(64/93),76株鲍曼不动杆菌经同源性分析得到院内泛耐药鲍曼不动杆菌的分布,最后,自建库对替加环素耐药鲍曼不动杆菌的正确检出率为87. 4%,替加环素敏感菌株的正确检出率为77. 5%。结论 MALDI-TOF MS可快速鉴定鲍曼不动杆菌、琼氏不动杆菌及医院不动杆菌,可对鲍曼不动杆菌进行同源性分析,通过自建库可预测鲍曼不动杆菌对替加环素的耐药性。Absrtact Objective To evaluate the identification effect, homology analysis ability and self-library effect of MALDI-TOF MS technology for Acinetobacter baumannii. Methods 107 strains of Acinetobacter baumannii were identified by MALDI-TOF MS technology, and then drug sensitivity analysis was carried out. Tigacycline-resistant and tigacycline-susceptible Acinetobacter baumannii were screened out. Databases of tigacycline-resistant and tigacycline-susceptible Acinetobacter baumannii were established using the screened Acinetobacter baumannii. Finally, self-built databases were verified. Results MALDI-TOF MS identified 107 strains of bacteria as 103 strains of Acinetobacter baumannii, 3 strains of Acinetobacter junii and 1 Acinetobacter nosocomium. K-B and broth dilution methods were used to detect the sensitivity of 103 strains of Acinetobacter baumannii to tegacycline. 37 strains of Acinetobacter baumannii resistant to tegacycline and 39 strains of Acinetobacter baumannii susceptible to tegacycline were obtained. The consistency rate of the methods was 68.82%(64/93). Homology analysis for 76 strains of Acinetobacter baumannii revealed the distribution of pan-drug-resistant Acinetobacter baumannii in hospital. For self-built databases, the correct detection rate of tigacycline-resistant Acinetobacter baumannii and tigacycline-sensitive Acinetobacter baumannii was 87.4% and 77.5% respectively. Conclusion MALDI-TOF MS can quickly identify Acinetobacter baumannii, Acinetobacter junii and Acinetobacter nosocomium, and it can carry out Homology analysis of Acinetobacter baumannii. The resistance of Acinetobacter baumannii to tegacycline can be predicted by self-built databases.

关 键 词：MALDI-TOF MS 替加环素 鲍曼不动杆菌 同源性分析 自建库 

分 类 号：R446.5[医药卫生—诊断学]