性别对咪喹莫特诱导小鼠银屑病模型建立的影响  

Effect of gender on the establishment of mouse psoriasis induced by imiquimod

作　　者：罗婷婷 韩陈陈 崔东倩 张雨 刘倩 马旸[1] 魏伟[1] Luo Tingting;Han Chenchen;Cui Dongqian(Institute op Clinical 2harmacology,Anhui MePkai Universith,Oep Laboratorf of Aoti-ifflammatorg and Immune MePicinp Ministrs op Educatioo,Anhui CoHaboratwe Innovahon Cefter cf Afti-ioflammators and Immune MePicinp,Hefei 230032)

机构地区：[1]安徽医科大学临床药理研究所、抗炎免疫药物教育部重点实验室、抗炎免疫药物安徽省协同创新中心,合肥230032

出　　处：《安徽医科大学学报》2020年第4期634-639,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81502123、81330081、81673444)。

摘　　要：建立咪喹莫特(IMQ)诱导的小鼠银屑病模型,对模型进行评价并探究性别对IMQ诱导小鼠银屑病模型建立的影响。BALB/c小鼠,雌雄各半,随机分为正常对照组、模型组1(雄性)、模型组2(雌性),将IMQ涂抹于小鼠背部皮肤建立小鼠银屑病模型。每天对小鼠皮肤进行银屑病皮损面积和严重程度(PASI)评分,HE染色观察皮肤组织病理学改变,计算脾脏指数、胸腺指数,免疫组化法检测增殖细胞核抗原(PCNA)的表达和免疫荧光法检测内披蛋白的表达情况。与对照组小鼠相比,IMQ诱导的雄性和雌性小鼠皮肤均表现出鳞屑、红斑和增厚现象并持续加重,PASI评分也呈相同的结果。HE染色发现与对照组小鼠相比,IMQ诱导的雄性和雌性小鼠表皮均增厚(P<0.001),表皮浅层可见毛细血管扩张、炎症细胞浸润、角质层可见角化不全、颗粒层变薄甚至消失、棘层肥厚、并有表皮突下延。IMQ诱导的小鼠脾脏指数增大(P<0.001),雌雄小鼠脾脏指数变化差异无统计学意义。IMQ诱导的雄性和雌性小鼠角质形成细胞表达数量增多(P<0.05),且存在异常分化现象,雌雄小鼠PCNA和内披蛋白的表达差异无统计学意义。IMQ诱导的小鼠银屑病模型与人类银屑病具有许多相似性,且性别对IMQ诱导小鼠银屑病模型的建立几乎没有影响。An imiquimod(IMQ)-induced psoriasis mouse model was established to evaluate the model and explore the effect of gender on IMQ-induced mouse psoriasis model establishment. Half of male and female BALB/c mice were randomly divided into normal control group,model group 1(male) and model group 2(female),and IMQ was applied to the back skin of mice to establish a mouse psoriasis model. The psoriasis area and severity index(PASI) was scored daily for mouse skin. The pathological changes of skin were observed by HE staining. The thymus index and spleen index were calculated. The expression of proliferating cell nuclear antigen(PCNA) was detected by immunohistochemistry and the expression of involucrin was detected by immunofluorescence. Compared with the control mice,IMQ-induced male and female mice showed scaly,erythema and thickening and continued to deteriorate,and the PASI score also showed the same results. HE staining found that compared with control mice,the epidermis of IMQ-induced male and female mice were thickened(P<0.001),telangiectasia and inflammatory cell infiltration were observed in the superficial epidermis. Parakeratosis was observed in the stratum corneum and the granular layer became thinner or even disappeared. The acanthosis was thick,and epidermis extended downward. The spleen index of IMQ-induced mice increased(P<0.001),and there was no significant difference in spleen index between male and female mice. The expression of keratinocytes in IMQ-induced male and female mice increased(P<0.05) and there was abnormal differentiation. There was no significant difference in the expression of PCNA and involucrin between male and female mice. The IMQ-induced mouse psoriasis model has many similarities with human psoriasis,and gender has little effect on the establishment of IMQ-induced mouse psoriasis model.

关 键 词：性别 银屑病 咪喹莫特 动物模型 

分 类 号：R965.1[医药卫生—药理学]