iTRAQ法分析原发性慢性闭角型青光眼患者血浆中蛋白组学变化  

Analysis of proteome in plasma of patients with primary chronic angle-closure glaucoma by iTRAQ

作　　者：李倩[1] 张立宇 蒋正轩[1] 沈兵[2] 许育新[1] Li Qian;Zhang Liyu;Jiang Zhengxuan(Dept of Ophthalmology,The Second Afiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院眼科,合肥230601 [2]安徽医科大学生理教研室,合肥230032

出　　处：《安徽医科大学学报》2020年第4期640-644,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究重点项目(编号:KJ2018A0664)。

摘　　要：通过分析原发性慢性闭角型青光眼(CPACG)患者血浆中蛋白组学的变化,找出血浆差异蛋白(DEPs),发现该病可能存在的血浆蛋白标志物。采集实验对象血浆,通过同位素标记相对与绝对定量技术(iTRAQ)标记蛋白,液相色谱串联质谱(LC-MS/MS)检测后对DEPs进行基因本体论分析(GO分析)和基于京都基因与基因组百科全书分析(KEGG分析),根据DEPs的主要作用途径找出关键蛋白。质谱分析共筛选出143个有统计学意义的DEPs(P<0.05),其中49个上调,94个下调。GO分析明确DEPs分子功能、生物过程和细胞组成3个部分的分布情况。KEGG分析显示DEPs主要与代谢通路和补体凝集联级反应有关,根据作用机制不同分为以下3类:①调节青光眼视神经节细胞相关蛋白:肝细胞生长因子激动剂(HGFA);②氧化应激相关蛋白:超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GPx-3)、过氧化物酶(Peroxiredoxin-2);③免疫反应相关蛋白:补体4(C4)、补体4结合蛋白(C4BP)。这些DEPs可能为临床诊断提供了新的血浆标志物,为明确CPACG的病理机制和临床诊断提供了新思路。In order to look for the plasma differential proteins(DEPs) and the possible plasma protein markers, we analyzed the proteomics in plasma of patients with primary chronic angle closure glaucoma. The plasma was collected, and iTRAQ and LC-MS/MS were performed to find the different proteins(DEPs). The DEPs were analyzed by GO and KEGG to find out the main pathways and key proteins. 143 statistically significant proteins were screened by mass spectrometry(P<0.05), of which 49 were up-regulated and 94 were down-regulated. The DEPs were analyzed from three parts such as the molecular function, biological process and cellular component by GO analysis. The main pathways are metabolic pathway and complement coagulation cascades,the key proteins are divided into the following three kinds according to their different functions:(1) regulating the glaucoma optic ganglion cells: HGFA;(2) oxidative stress related: glutathione peroxidase 3,SOD,Peroxiredoxin-2;(3) immune response related: C4,C4 BP. These differential proteins provide new plasma markers for clinical diagnosis. It helps us to understand the pathological mechanism of primary angle closure glaucoma and provides a new idea for clinical diagnosis.

关 键 词：CPACG iTRAQ法 LC-MS/MS 蛋白组学 

分 类 号：R320.37[医药卫生—人体解剖和组织胚胎学]