桥本甲状腺炎鼠动情周期子宫内膜半乳糖凝集素-1含量变化  

Changes of galectin-1 content in endometrium of Hashimoto thyroiditis rats during estrous cycle

作　　者：高伟伟 李莉[1] 樊华 杨昊[1] 柳田田 王囡[1] 朱德发[1] Gao Weiwei;Li Li;Fan Hua(Dept of Geriatric Endocinology,The First Afiliated Hospital of Anhui Medical University,Hefei 230022;Dept of General Internal Medicine,The Second People's Hospital of Anhui,Hefei 230041)

机构地区：[1]安徽医科大学第一附属医院老年内分泌科,合肥230022 [2]安徽省第二人民医院普内科,合肥230041

出　　处：《安徽医科大学学报》2020年第3期344-348,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272152)。

摘　　要：目的研究桥本甲状腺炎鼠动情周期子宫内膜半乳糖凝集素-1(galectin-1)表达。方法选取8~9周龄的雌性小鼠共40只,按照随机数字表法将其均分成观察组和对照组。其中观察组小鼠于尾根部予以猪甲状腺球蛋白皮下注射,建立桥本甲状腺炎模型。分别比较2组小鼠血清甲状腺球蛋白抗体(TgAb)、甲状腺过氧化物酶抗体(TPOAb)、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)、促甲状腺激素(TSH)水平,动情各期的子宫内膜厚度、galectin-1 mRNA表达情况、galectin-1蛋白的平均吸光度值(AOD)、子宫组织匀浆肿瘤坏死因子-α(TNF-α)和白细胞介素-17a(IL-17a)水平。结果与对照组相比,观察组小鼠血清TgAb、TPOAb水平增高(P<0.05),甲状腺激素水平差异无统计学意义(P>0.05);动情期子宫内膜厚度增加(P<0.05),galectin-1蛋白的表达减少(P<0.05),动情周期galectin-1 mRNA水平减低(P <0. 05),动情周期子宫组织匀浆中TNF-α、IL-17a表达减少(P <0. 05)。结论桥本甲状腺炎鼠动情期子宫内膜增厚,galectin-1含量减低。子宫组织匀浆内TNF-α和IL-17a两种细胞因子减少。Objective To study the expression of galactose agglutinin-1(galectin-1) in endometrium of Hashimoto’s thyroiditis rats during estrous cycle. Methods A total of 40 female mice aged 8~9 weeks were selected and divided into observation group and control group by random number table method, with 20 mice in each group. In the observation group, pig thyroglobulin was injected subcutaneously at the tail root to establish Hashimoto’s thyroiditis model. Mice serum thyroglobulin antibodies(TgAb), thyroid peroxidase antibody(TPOAb), triiodothyronine(T3),tetraiodothyronine(T4), thyroid stimulating hormone(TSH) level of two groups were compared respectively. The endometrial thickness,galectin-1 mRNA expression, and the average optical density(AOD) of galectin-1 protein in each period of estrus cycle were also compared. The levels of tumor necrosis factor-α(TNF-α) and interleukin-17 A(IL-17 A) were compared between the two groups. Results Compared with the control group, serum TgAb and TPOAb levels in the observation group increased(P<0.05), and there was no statistically significant difference in thyroid hormone levels(P>0.05). In the estrous phase, the endometrial thickness increased(P<0.05) while the expression of galectin-1 protein decreased(P<0.05). The mRNA level of galectin-1 decreased(P<0.05) during the estrous cycle, and the expressions of TNF-α and IL-17 a in the uterine tissue homogenate decreased(P<0.05). Conclusion The endometrium of Hashimoto’s thyroiditis rats becomes thicker and the content of galectin-1 decreases during estrus. Uterine tissue homogenate TNF-α and IL-17 a decrease.

关 键 词：桥本甲状腺炎 半乳糖凝集素-1 子宫内膜 动情周期 细胞因子 

分 类 号：R581.9[医药卫生—内分泌]