炎症和非炎症环境下肝干细胞细胞学特性观察  

Cytology research of liver stem cells in inflammatory and non-inflammatory environments

作　　者：黄俊凯 王艳 杨奉天 李婉 陈向金 杨柳 徐丽红[1,3] Huang Junkai;Wang Yan;Yang Fengtian(Dept of Gastroenterology,The First Affiliated Hospital of The Medical College,Shihezi University,Shihezi 832008;Kaifeng Central Hospital Endoscopy Center,Kaifeng 475100)

机构地区：[1]石河子大学医学院第一附属医院消化内科,石河子832008 [2]开封市中心医院内镜中心,开封475100 [3]成都中医药大学附属第五人民医院消化内科,成都611130

出　　处：《安徽医科大学学报》2020年第3期373-378,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81360076)。

摘　　要：目的通过构建炎症和非炎症肝干细胞增殖模型,观察肝干细胞在炎症增殖环境和非炎症增殖环境中的细胞学特性差异。方法以2-乙酰氨基芴+肝大部切除术构建非炎症环境肝干细胞增殖模型,以2-乙酰氨基芴+四氯化碳构建炎症环境肝干细胞增殖模型。苏木精-伊红(HE)染色观察肝脏组织结构变化;免疫组化法观察肝干细胞EpCAM、CD133、OV6的表达,运用Image-Pro Plus 6.0软件分析平均吸光度值;透射电镜观察肝干细胞超微结构变化。结果成功建立炎症、非炎症环境肝干细胞增殖模型。HE染色:非炎症环境肝干细胞增殖模型大鼠肝脏未见炎性细胞;炎症环境肝干细胞增殖模型大鼠肝脏见大量炎性细胞。免疫组化:EpCAM、CD133、OV6表达阳性的肝干细胞均可在炎症和非炎症环境肝干细胞增殖模型中被发现,炎症环境肝干细胞增殖模型EpCAM、CD133、OV6表达量较非炎症环境肝干细胞增殖模型高(P<0.05)。透射电镜:两种肝干细胞增殖模型中均可见3型肝干细胞亚型;炎症环境增殖模型异型肝干细胞数较非炎症环境增殖模型多(P<0.05)。结论较非炎症肝干细胞增殖环境,炎症环境中EPCAM^+、CD133^+、OV6^+的肝干细胞增多,肝干细胞超微结构的异常可能在炎症早期就已出现。Objective To observe cytology differences of liver stem cells(LSCs) in non-inflammatory proliferation environment and inflammatory proliferation environment by establishing non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model. Methods 2-acetaminofluorenced combined two thirds partial hepatectomy protocol was established as non-inflammatory LSCs proliferation model. 2-acetaminofluorence combined carbon tetrachloride protocol was established as inflammatory LSCs proliferation model. Liver histological changes were observed by hematoxylin and eosin staining. The expressions of EpCAM, CD133 and OV6 of LSCs were detected by immunohistochemistry, and average optical density values were measured by Image-Pro Plus 6.0. The ultrastructure changes of LSCs were observed by transmission electron microscopy. Results Non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model were established successfully. HE staining:non-inflammatory LSCs proliferation model rats’ liver were absent with inflammatory cells infiltration;inflammatory LSCs proliferation model rats’ liver were fibrosis formation period with large number of inflammatory cells infiltration. Immunohistochemical: EpCAM-positive, CD133-positive, and OV6-positive LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, the expression levels of EpCAM, CD133, OV6 of inflammatory LSCs proliferation model were significantly higher than those of non-inflammatory LSCs proliferation model(P<0.05). Transmission electron microscopytype: Ⅰ,Ⅱ, and Ⅲ of LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, however, there were more heteromorphic LSCs in inflammatory LSCs proliferation model than those in non-inflammatory LSCs proliferation model(P<0.05). Conclusion There are more EpCAM^+, CD133^+, OV6^+ LSCs in inflammatory LSCs proliferation model compared with non-inflammatory LSCs proliferation model, and there are LSCs with abnormal subcellular structure in t

关 键 词：肝干细胞 炎症环境 亚细胞结构 

分 类 号：R575.1[医药卫生—消化系统]