乙型病毒性肝炎患者血浆中miR-122及miR-155动态表达及意义  

Dynamic expression and significance of miR-122 and miR-155 in plasma of patients with viral hepatitis type B

作　　者：李雪 王悦 张扬[4] 蔡海峰[4] 冯福民 张美航 吴冬雪 王雪 张咪 朱晗瑀 陈琦 杨帆 Li Xue;Wang Yue;Zhang Yang(School of Public Health,North China University of Science and Technology,Tangshan 063000;School of Economics,North China University of Science and Technology,Tangshan 063000;Dept of Medical Record,North China University of Science and Technology Affiliated Hospital,Tangshan 063000;Tangshan People's Hospital,Tangshan 063000)

机构地区：[1]华北理工大学公共卫生学院,唐山063000 [2]华北理工大学经济学院,唐山063000 [3]华北理工大学附属医院病案科,唐山063000 [4]唐山市人民医院,唐山063000 [5]华北理工大学医学实验研究中心,唐山063000 [6]华北理工大学人事处,唐山063000

出　　处：《安徽医科大学学报》2020年第3期447-450,共4页Acta Universitatis Medicinalis Anhui

基　　金：河北省研究生创新资助项目(编号:2016198)。

摘　　要：目的动态观察乙肝患者血浆中miR-122、miR-155的表达并分析其与炎症因子、细胞周期因子的相关性。方法实时荧光定量PCR(qRT-PCR)检测两组血浆中miR-122、miR-155的表达水平;ELISA检测炎症因子白细胞介素(IL)-6,肿瘤坏死因子(TNF)-α和细胞周期因子p53、CyclinD1的表达水平。结果乙肝患者血浆中miR-122、miR-155均明显升高(P<0.05);在继续治疗的过程中,患者血浆中miR-122先升高再下降,miR-155表达下降。miR-122、miR-155的表达与丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、IL-6、TNF-α、细胞周期因子CyclinD1等均呈正相关(P<0.01);其中miR-155与其相关性更明显;同时miR-155与p53呈负相关(P<0.01)。结论乙型病毒性肝炎患者血浆中miR-122和miR-155的异常表达与乙型肝炎所致肝损伤有关,为乙型病毒性肝炎导致的肝损伤的监测及治疗提供了新思路。Objective To observe the expression of miR-122 and miR-155 in plasma of patients with viral hepatitis type B and analyze their correlation with inflammatory factors and cell cycle factors. Methods The expressions of miR-122 and miR-155 were determined by qRT-PCR. The expressions of interleukin(IL)-6, tumor necrosis factor(TNF)-α, P53, CyclinD1 were determined by ELISA. Results The results of qRT-PCR showed that expressions of miR-122 and miR-155 in the plasma of patients with viral hepatitis type B were high(P<0.05). During the course of continuous treatment, miR-122 in the patient’s plasma increased first and then decreased, and miR-155 expression decreased. The expressions of miR-122 and miR-155 were positively correlated with alanine aminotransferase(ALT),aspartate aminotransferase(AST), IL-6, TNF-α and CyclinD1(P<0.01), and miR-155 had more obvious correlation. There was a negative correlation with p53(P<0.01). Conclusion The abnormal expression of miR-122 and miR-155 in plasma of patients with hepatitis B virus(HBV) is related to liver damage caused by HBV, which provides a new idea for the monitoring and treatment of liver damage caused by HBV.

关 键 词：乙型病毒性肝炎 非编码RNA 肝损伤 炎症因子 细胞周期因子 

分 类 号：R512.62[医药卫生—内科学]