中药干预下脾虚痰浊动脉粥样硬化巴马猪小肠差异蛋白组学及生物信息学分析  

Differential proteomics and bioinformatics analysis of small intestine of atherosclerosis Bama pig with spleen deficiency and phlegm turbid under TCM intervention

作　　者：王佳[1] 贾连群[1] 宋囡[1] 马艺鑫 张立德[1] 张哲[2] 张会永[2] 杨关林[1] Wang Jia;Jia Lianqun;Song Nan(Liaoning University of Traditional Chinese Medicine,Shenyang 110847)

机构地区：[1]辽宁中医药大学,沈阳110847 [2]辽宁中医药大学附属医院,沈阳110032

出　　处：《安徽医科大学学报》2020年第5期756-762,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家重点基础研究计划(“973”计划)项目(编号:2013CB531704);辽宁省中央引导地方科技发展专项项目(编号:2018416016)。

摘　　要：目的应用iTRAQ技术研究中药干预下脾虚痰浊动脉粥样硬化(AS)巴马猪小肠差异蛋白组学的表达影响。方法10只健康半岁雄性广西巴马小型猪,随机均分为模型组、中药治疗组。分离提取小肠总蛋白,以iTRAQ试剂标记后进行质谱检测并以软件分析差异表达的蛋白质,运用LCESI-MS/MS方法分析模型组和治疗组的蛋白组学差异;对差异蛋白进行GO功能注释、富集和KEGG通路富集并筛选出显著富集的蛋白。结果治疗组与模型组共筛选出差异蛋白189种,其中70个上调蛋白和119个下调蛋白。GO富集分析显示,这些差异蛋白主要富集于绑定、催化活性、运输活性、结构分子活性等生物过程;细胞、细胞组分、细胞器、细胞膜、胞外区等细胞成分;细胞过程、生物调节、生物过程调节、代谢过程、刺激应答、定位等分子功能。KEGG通路分析显示,细胞黏附分子通路变化最为显著,吞噬体、突出囊泡循环、氨基酸生物合成、胰岛素分泌、PPAR信号通路以及与脂代谢密切相关的脂肪消化和吸收、胆汁酸分泌通路显著变化。此外,与能量代谢密切相关的氧化磷酸化等通路蛋白亦发生改变。结论中药干预下脾虚痰浊AS巴马猪小肠差异蛋白组学改变可能与细胞炎症反应、甘油脂类代谢、胆汁酸分泌等密切相关,脾虚高脂状态小肠消化吸收功能异常,细胞炎症反应可能与AS形成密切相关,中药干预治疗靶点可能在以上方向。Objective To study the effect of TCM intervention on differential proteomics of small intestine in atherosclerosis(AS)bama pig with spleen deficiency and phlegm turbid by iTRAQ technique.Methods Ten healthy half-year-old male Guangxi Bama miniature pigs were randomly divided into model group and TCM group,five pigs in each group.Total proteins of small intestine was isolated,labeled with iTRAQ reagent and detected by mass spectrometry.The differentially expressed proteins were analyzed by software,and the proteomics differences between the model group and the treatment group were analyzed by LC-ESI-MS/MS method.The significantly enriched proteins were selected GO function annotation,enrichment and KEGG pathway enrichment.Results A total of 189 different proteins were screened in the treatment group compared with the model group,including 70 up-regulated proteins and 119 down-regulated proteins.GO enrichment analysis showed that these differential proteins were mainly concentrated in biological processes such as binding,catalytic activity,transport activity and structural molecular activity.It was concentrated in cells,cell components,organelles,cell membranes and extracellular regions.The molecular functions such as cell process,biological regulation,biological process regulation,metabolic process,stimulation response and localization were enriched.Analysis of the KEGG pathway showed that cell adhesion molecular pathways had the most significant changes,with significant changes in phagocytosis,prominent vesicle circulation,amino acid biosynthesis,insulin secretion,PPAR signaling pathways,fat digestion and absorption,and bile acid secretion pathways closely related to lipid metabolism.In addition,oxidative phosphorylation and other pathway proteins closely related to energy metabolism also changed.Conclusion The differential proteomics changes of small intestine in bama pig with spleen deficiency and phlegm turbid under the intervention of TCM may be closely related to cellular inflammatory response,lipid metaboli

关 键 词：脾虚痰浊 动脉粥样硬化 ITRAQ 小肠 蛋白组学 

分 类 号：R2[医药卫生—中医学]