木薯ERF转录因子调控的靶基因筛选与表达分析  

Characterization and Expression Analysis of the Target Genes Regulated by ERF in Cassava

作　　者：宋娜 张刘宁颖 曹敏 郭文雅 洪雨慧 吴金山[1] 陈银华[1] 于晓惠 SONG Na;ZHANG Liuningying;Cao Min;GUO Wenya;HONG Yuhui;WU Jinshan;CHEN Yinhua;YU Xiaohui(College of Tropical Ccrops,Hainan University,Haikou,Hainan 570228;School of Life and Pharmaceutical Sciences,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带作物学院,海口570228 [2]海南大学生命科学与药学院,海口570228

出　　处：《热带生物学报》2020年第2期177-189,共13页Journal of Tropical Biology

基　　金：国家自然科学基金(31560497);海南省创新团队项目(2018CXTD330)。

摘　　要：为解析乙烯响应因子(ERF,Ethylene Response Factor)在木薯(Manihot esculenta)生长发育过程中的调控通路,本研究从木薯全基因组数据库中,筛选出启动子区含有GCC-box顺式作用元件的基因204个,利用生物信息学手段对这些基因进行染色体位置分布分析、病原菌侵染下表达模式分析以及启动子结构预测,部分候选基因的qRT-PCR结果表明:Manes.02G189600,Manes.03G039700,Manes.06G002000,Manes.09G063700,Manes.14G141600,Manes.15G181900可能参与调控植物的抗病途径,同时在乙烯介导的信号传递途径中也起重要作用。The defense response of plants to stress is largely regulated by the level of gene transcription,and the recognition and combination of the transcription factor and cis-acting elements on the target gene promoter plays a key regulatory role.ERF is a type of transcription factor that plays an important role in the growth and development of plants.There is a conserved GCC-box in the promoter region of downstream target genes regulated by it.In order to analyze the regulatory pathway of ERF in the growth and development of cassava,204 genes containing GCC-box cis-acting elements in the promoter region were screened.Chromosomal position distribution,analysis of expression patterns under pathogen infection,and promoter structure prediction of these genes were analyzed using bioinformatics methods.The qRT-PCR results showed that Manes.02G189600,Manes.03G039700,Manes.06G002000,Manes.09G063700,Manes.14G141600,Manes.15G181900 might be involved in regulating plant disease resistance pathways,and play an important way in ethylene-mediated signal transduction pathways.

关 键 词：木薯 GCC-BOX ERF转录因子 生物胁迫 

分 类 号：S188.2[农业科学—农业基础科学] S533