转录调节因子VrhR负调控野油菜黄单胞菌的致病力  

The Transcriptional Regulator VrhR Negatively Regulates the Virulence of Xanthomonas campestris pv.campestris

作　　者：张钰[1,2] 林茂娟 李婷[1,2] 李春霞[2] 陈银华[2] 陶均[2] ZHANG Yu;LIN Maojuan;LI Ting;LI Chunxia;CHEN Yinhua;TAO Jun(School of Life Science and Pharmacy,Hainan University,Haikou,Hainan 570228,China;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学生命科学与药学院,海口570228 [2]海南大学海南省热带生物资源可持续利用重点实验室/热带作物学院,海口570228

出　　处：《热带生物学报》2020年第2期217-222,237,共7页Journal of Tropical Biology

基　　金：海南大学科研启动基金(kyqd1546);海南省自然科学基金(20163049;20163050)。

摘　　要：以野油菜黄单胞菌(Xanthomonas campestris pv.campestris,Xcc)转录调节因子VrhR,VrhA和VrhB为研究对象,通过基因敲除和致病性分析确定它们在Xcc与宿主互作过程中的作用。结果表明:VrhR只含有HTH(helix-turn-helix)结构域;在基因组上vrhR位于其他2个lysR类转录调节因子(vrhA和vrhB)之间,但转录方向相反。vrhR突变会提高Xcc的致病能力,但vrhA和vrhB突变对病原菌的致病能力没有影响。同时,vrhR,vrhA和vrhB突变均不影响细菌的运动性及胞外酶(蛋白酶、纤维素酶和淀粉酶)的活性。The roles of transcriptional regulators,VrhR,VrhA and VrhB,in the interaction between Xanthomonas campestris pv.campestris and plants were analyzed via gene mutation and virulence analysis.The results showed that VrhR contains only one HTH(helix-turn-helix)motif and is located between the other two LysR transcriptional regulators(vrhA and vrhB)in Xcc 8004 genome,but transcribed in an opposite direction.Mutation of vrhR increased the pathogenicity of Xcc,while mutation of vrhA and vrhB did not affect Xcc virulence.However,mutation of vrhR,vrhA and vrhB did not affect bacterial motility and extracellular enzyme(protease,cellulase and amylase)activities,suggesting that vrhR may regulate bacterial virulence by regulating other pathogenic factors.

关 键 词：野油菜黄单胞菌 HTH转录调节因子 VrhR 致病性 

分 类 号：S43.42[农业科学—农业昆虫与害虫防治] Q756[农业科学—植物保护]