瞬时遗传转化长白落叶松NAC基因植株抗旱性的研究  

Drought Resistance of Larch NAC Gene by Transient Genetic Transformation

作　　者：张磊[1] 熊欢欢 曹庆 赵佳丽[1] 张含国[1] ZHANG Lei;XIONG Huan-Huan;CAO Qing;ZHAO Jia-Li;ZHANG Han-Guo(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2020年第3期394-400,共7页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31700595);国家科技重大专项课题(2018ZX08020-003);中央高校基本科研业务费专项资金项目(2572019BA13)。

摘　　要：通过对长白落叶松转录组分析,克隆到1条NAC转录因子命名为LoNAC18,序列分析结果表明:基因全长1 101 bp,编码366个氨基酸,为不稳定亲水性蛋白。该基因瞬时遗传转化的长白落叶松植株在PEG模拟干旱胁迫条件下与对照相比,基因表达量上调,SOD、POD、可溶性蛋白、可溶性糖含量增加,MDA含量低于对照植株,说明LoNAC18基因在一定程度上参与调控长白落叶松应答PEG模拟干旱胁迫的过程,为进一步研究NAC基因在针叶树种胁迫应答中的功能与作用机制提供了参考依据。An NAC transcription factor named LoNAC18 was cloned by transcriptome analysis of Larix olgensis.The sequence analysis results showed that the whole gene was 1 101 bp,encoding 366 amino acids,and it was an unstable hydrophilic protein. Compared with the control,gene expression increased of the transient genetic transformation plant under PEG simulated drought stress,while SOD,POD,soluble protein,soluble sugar content was all increased,and the MDA content is lower than the control plants. Therefore,LoNAC18 genes was involved in regulating the L. olgensis response to PEG stress,the results could provide the reference to research on NAC function and mechanism of action of conifer stress response.

关 键 词：长白落叶松 瞬时遗传转化 NAC PEG 

分 类 号：S791.22[农业科学—林木遗传育种]