华重楼叶斑病的病原菌鉴定与室内药剂筛选  

Identification of the Pathogen of Leaf Spot of Paris polyphylla Smith var.chinensis(Franch.)Hara.and Fungicide Screening in Laboratory

作　　者：克永霞 伏荣桃[2] 刘圆[3] 卢代华[2] 陈诚[2] 王剑[2] KE Yong-xia;FU Rong-tao;LIU Yuan;LU Dai-hua;CHEN Cheng;WANG Jian(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Institute of Plant Protection,Sichuan Academy of Agricultural Science,Chengdu 610066,China;Ethnic Medicine Institute,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学药学院,成都610041 [2]四川省农业科学院植物保护研究所,成都610066 [3]西南民族大学民族医药研究院,成都610041

出　　处：《西南大学学报（自然科学版）》2020年第5期63-68,共6页Journal of Southwest University(Natural Science Edition)

基　　金：四川省科技计划项目(2018JY0111);国家“十三五”科技支撑计划项目(2015BAC05B02);西南民族大学2018年研究生创新型科研项目(CX2018SZ83).

摘　　要：四川地区人工种植华重楼每年5-7月常发生叶斑病害,严重危害华重楼的质量和产量.该试验采用组织分离法对华重楼病原菌进行分离与鉴定,并采用菌丝生长速率测定法对病原菌进行了室内11种药剂毒力测定.结果表明:确定病原菌为细交链孢菌Alternaria tenuis Nees.11种药剂对病原菌都有不同程度的抑制作用,同药剂之间抑菌效果有差异.其中,10%多抗霉素可湿性粉剂、80%乙蒜素乳油、68%宁南霉素水剂3种药剂的抑菌效果最好,EC 50值分别为11.921 mg/L,13.511 mg/L,25.772 mg/L;2%春雷霉素水剂、5%氨基寡糖素水剂、3%中生菌素可湿性粉剂3种药剂的抑菌效果较好,EC 50值分别为11.921 mg/L,13.511 mg/L,25.772 mg/L;50%氯溴异氰尿酸可溶粉剂、30%己唑醇悬浮剂、75%肟菌·戊唑醇水分散粒剂、0.5%几丁聚糖水剂4种药剂的抑菌效果一般,EC 50值分别为48.08 mg/L,54.169 mg/L,65.939 mg/L,76.521 mg/L;20%井冈霉素水溶粉剂的抑菌效果最差,EC 50值为131.737 mg/L.根据室内毒力测定表明,10%多抗霉素可湿性粉剂、80%乙蒜素乳油、68%宁南霉素水剂3种药剂的抑菌效果最好.Leaf spot frequently occurs from May to August in the planting areas of Paris polyphylla Smith var.chinensis(Franch.)Hara in Sichuan and causes serious damage to the quality and yield of the crop.In this experiment,the method of tissue isolation was used to isolate and identify the pathogen of the disease and the method of mycelia growth rate determination was used to determine the toxicity of 11 drugs in laboratory.The pathogenic fungus was identified as Alternaria tennis Nees.The toxicity test showed that all the drugs studied had,in different degrees,inhibitory effect on the pathogen,and that the bacteriostatic effect was different between potions.Of all the treatments,10%polyoxin WP,80%ethylicin EC and 68%Ningnanmycin AS gave the best inhibitory effect,their EC50 values being 11.921,13.511 and 25.772 mg/L,respectively;the bacteriostatic effects of 2%Kasugamycin AS,5%amino Oligosaccharin AS and 3%medium bioactinomycin WP were satisfactory,with an EC50 value of 11.921,13.511 and 25.772 mg/L,respectively.The effects of 50%chloro brominated isocyanuric acid SP,30%hexaconazole SC,75%oxime tebuconazole WG and 0.5%chitosan AS were acceptable,their EC50 values being 48.08,54.169,65.939 and 76.521 mg/L,respectively.20%Jiangangmycin AF had the poorest inhibitory effect,its EC50 value being 131.737mg/L.According to the laboratory test,10%polyoxin WP,80%ethylicin EC and 68%Ningnanmycin AS have the best fungus-inhibiting effect,and a field experiment is being made to verify these results.

关 键 词：华重楼 叶斑病 细交链孢菌 药剂筛选 

分 类 号：S432[农业科学—植物病理学]