Express and sensitive detection of multiple miRNAs via DNA cascade reactors functionalized photonic crystal array  

作　　者：Yingfei Wang Yuyi Li Yue Zhang Kewei Ren Huangxian Ju Ying Liu 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China [2]Chemistry and Biomedicine Innovation Center,Nanjing University,Nanjing 210023,China [3]Department of Chemistry,University of Massachusetts,Amherst,Massachusetts 01003,USA

出　　处：《Science China Chemistry》2020年第5期731-740,共10页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21635005,21605083,21974064);the National Research Foundation for Thousand Youth Talents Plan of China,Specially-appointed Professor Foundation of Jiangsu Province,Program for innovative Talents and Entrepreneurs of Jiangsu Province。

摘　　要：Array based detection techniques with fluorescence signal reading is a powerful tool for multiple targets analysis. However,when applied fluorescence array for micro RNA detection, time-consuming multi-steps surface signal amplification is usually required due to the low abundance of micro RNA in total RNA expressions, which impairs detection efficiency and limits its application in point of care test(POCT) manner. Herein, DNA cascade reactors(DCRs) functionalized photonic crystal(PC)array was fabricated for express and sensitive detections of mi RNA-21 and mi RNA-155. DCRs were assembled by interval conjugation of self-quenched hairpin DNA probes to single strand DNA nanowire synthesized by rolling circle amplification,which generated cascade DNA hybridization reactions in response to target mi RNAwith instant fluorescence recovery signal. PC array patterns with multi-structure colors further amplified fluorescence with their respective photonic bandgaps(PBGs)matching with the emission peaks of fluorescence molecules labelled on DCRs. The as-prepared DCRs functionalized PC array demonstrated express and sensitive simultaneous detections of mi RNA-21 and mi RNA-155 with hundreds f M detection limits only in 15 min, and was successfully applied in fast quantifications of low abundance mi RNAs from cell lysates and spiked mi RNAs from human serum, which would hold great potential for disease diagnosis and therapeutic effect monitoring with a POCT manner.

关 键 词：micro RNA detection photonic crystal array DNA cascade reactors fluorescence amplification express detection 

分 类 号：Q503[生物学—生物化学] O734[理学—晶体学] O657.3