过表达番茄LeDnaJ基因提高陆地棉(Gossypium hirsutum Linn.)R15的耐盐性  

Investigation on the improvement of salt tolerance in Gossypium hirsutum Linn.R15 through overexpression of tomato LeDnaJ gene

作　　者：闻甜 陈祥龙 武晓刚 权永刚 徐鹏[2] 郭琪[2] 倪万潮[2] 陈爱民 WEN Tian;CHEN Xiang-long;WU Xiao-gang;QUAN Yong-gang;XU Peng;GUO Qi;NI Wan-chao;CHEN Ai-min(Join Hope Seeds Co.,Ltd./Key Laboratory for the Creation of Cotton Varieties in the Northwest,Ministry of Agriculture and Rural Affairs,Changji 831100,China;Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China)

机构地区：[1]九圣禾种业股份有限公司/农业农村部西北内陆棉花品种创制重点实验室,新疆昌吉831100 [2]江苏省农业科学院经济作物研究所/农业农村部长江下游棉花油菜重点实验室,江苏南京210014

出　　处：《江苏农业学报》2020年第2期271-276,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家转基因生物新品种培育科技重大专项(2018ZX08009-15B)。

摘　　要：DnaJ蛋白不仅在生物体应对热激胁迫方面起作用,而且能响应盐、重金属和氧化等多种胁迫,因此,DnaJ蛋白的相关研究对植物抗逆研究具有重要意义。在前期的研究中,笔者所在课题组已经利用电子克隆及逆转录-聚合酶链式反应(RT-PCR)的方法从番茄中克隆了全长为465 bp的热激蛋白基因LeDnaJ。为了进一步验证其功能,针对LeDnaJ基因构建了含有35S启动子及NOS终止子的植物表达载体,通过农杆菌介导法转化到陆地棉(Gossypium hirsutum Linn.)品系R15中。目的基因的PCR检测结果表明,LeDnaJ基因已经整合到陆地棉R15基因组中;耐盐性鉴定结果表明,LeDnaJ基因的表达,提高了陆地棉R15萌发期、苗期的耐盐性。该研究结果为棉花耐盐性的改良提供了新的基因资源。DnaJ protein not only plays a role in the response of organisms to heat shock stress,but also responds to various stresses such as salt,heavy metals and oxidation,so the related researches are of great significance to stress resistance investigations in plants.The heat shock protein gene LeDnaJ with a whole length of 465 bp was cloned from tomato by the methods of electronic cloning and reverse transcription-polymerase chain reaction(RT-PCR)in the previous studies.In order to verify the functions of LeDnaJ gene,the plant expression vector containing LeDnaJ gene with NOS terminator and 35S promoter was constructed and transformed into Gossypium hirsutum Linn.R15 by Agrobacterium-mediated method.The PCR detection result of the target gene showed that the LeDnaJ gene had been integrated into the cotton genome.The results of salt tolerance identification indicated that the expression of LeDnaJ gene improved the salt tolerance of Gossypium hirsutum Linn.R15 at germination and seedling stages.These results provide new genetic resources for the improvement of salt tolerance in cotton.

关 键 词：陆地棉 LeDnaJ基因 过表达 耐盐性 

分 类 号：S332.6[农业科学—作物遗传育种]