结肠腺癌患者血清miR-551b-3p和miR-143表达水平及临床意义  

Value of serum levels of miR-551b-3p and miR-143 in the patients with colonic adenocarcinoma

作　　者：尹鹏[1] 胡君[1] 储著凌[1] 栾荣刚[1] 胡国强[1] 张佳骥 吉涛 张维[2] YIN Peng;HU Jun;CHU Zhulin;LUAN Ronggang;HU Guoqiang;ZHANG JiaJi;JI Tao;ZHANG Wei(General Surgery and Plastic Surgery Department, 454th Hospital of PLA, Nanjing 210002, China;Respiratory Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China)

机构地区：[1]解放军第四五四医院普外烧伤整形外科,江苏南京210002 [2]成都医学院第一附属医院呼吸内科,四川成都610000

出　　处：《西部医学》2020年第5期726-729,共4页Medical Journal of West China

基　　金：国家自然科学基金青年科学基金(81600388);南京军区科研课题面上项目(15MS072)。

摘　　要：目的探讨结肠腺癌患者血清miR-551b-3p和miR-143的表达水平及临床意义。方法选取2018年1月~2019年4月解放军第四五四医院手术活检病理确诊的87例结肠腺癌患者作为病例组,选取同期结肠息肉患者106例作为对照组,记录患者基本信息并使用qPCR方法对两组患者循环血中miR-551b-3p和miR-143表达水平进行检测,并与结肠腺癌的分期和分级的关系进行分析。结果病例组患者循环血中miR-551b-3p和miR-143水平明显低于对照组患者(P均<0.05);结肠腺癌分化差、TNM分期晚、有淋巴结转移和远处转移的患者循环血中miR-551b-3p和miR-143水平下降更加明显(P均<0.05)。结论miR-551b-3p和miR-143可能参与了结肠腺癌肿瘤的增殖、分化及转移过程,循环血中miR-551b-3p和miR-143有望成为诊断和预测结肠腺癌预后的指标,但仍需扩大样本量进一步研究证实。Objective To explore the value of serum levels of miR-551b-3p and miR-143 in the diagnosis and prognosis of colonic adenocarcinoma.Methods In this clinical study,87 patients with colonic adenocarcinoma(Observation group)and 106 patients with colonic polyps(Control group)were included.The fundamental and basic information were recorded.qPCR was used to analyze miR-551b-3p and miR-143 expressions in serum.Correlation between the serum level of miR-551b-3p and miR-143,and clinic pathological features in the patients with colonic adenocarcinoma were analyzed.Results The serum level of miR-551b-3p and miR-143 were markedly lower in patients with colonic adenocarcinoma than in colonic polyps(P<0.05 in all).The levels of miR-551b-3p and miR-143 were negatively correlated with tumor differentiation,TNM stages,lymph node metastasis,and distant metastasis in patients with colonic adenocarcinoma(P<0.05 in all).Conclusion In our study,the results indicated that miR-551b-3p and miR-143 probably involved in the proliferation,differentiation,and metastasis of colonic adenocarcinoma.Thus,miR-551b-3p and miR-143 are the potential biomarkers in the diagnosis and prognosis of colonic adenocarcinoma.However,the large scale clinical investigation still needs to be implemented to confirm their value in the future.

关 键 词：结肠腺癌 结肠息肉 miR-551b-3p MIR-143 TNM分期 

分 类 号：R473.73[医药卫生—护理学]