pH/退火方式控制DNA纳米笼的选择性组装  

Selective assembly of DNA nanocages controlled by pH/annealing

作　　者：叶超 曹行行 张荣荣 YE Chao;CAO Hang-hang;ZHANG Rong-rong(School of Chemistry and Chemical Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]合肥工业大学化学与化工学院,安徽合肥230009

出　　处：《现代化工》2020年第4期179-183,共5页Modern Chemical Industry

基　　金：中央高校基本科研业务费(JZ2016HGPA0734)。

摘　　要：通过调控溶液的pH、选择不同速率热退火方式,以四点星DNA分子瓦为建筑模块,根据沃森-克里克碱基互补配对原则和Hoogsteen氢键配对连接,选择性地组装成不同的DNA多面体结构。非变性聚丙烯酰胺凝胶电泳表征结果表明,在酸性条件(pH 5. 5)及退火时间5. 0 h下,分子瓦自组装成DNA变形四面体(tTET)结构;在中性条件(pH 8. 0)及退火时间24. 0 h下,自组装形成DNA八面体(OCT)结构。在中性条件(pH 8. 0)下,只改变分子瓦黏性末端碱基的数目,直至形成不同数目的碱基对来产生不同强度的作用力,也能类似地组装成不同的DNA多面体结构。该结果也由非变性聚丙烯酰胺凝胶电泳进行了表征。By regulating p H value of the solution and selecting different speeds of thermal annealing methods,fourpoint-star DNA molecular tiles are selectively assembled into different DNA polyhedral structures according to WatsonCrick base complementary pairing principle and Hoogsteen hydrogen bond pairing connection. Non-denaturing polyacrylamide gel electrophoresis( PAGE) shows that the tiles can be self-assembled into a DNA transmutative tetrahedron( tTET) under acidic condition( pH = 5. 5) for 5. 0 h of annealing. DNA octahedra( OCT) can be formed under neutral condition( pH = 8. 0) for 24. 0 h of annealing. Going further and changing only the number of sticky end bases of tiles under neutral condition( pH = 8. 0),they can form different numbers of base pairs with different strengths,which can be similarly assembled into different DNA polyhedral structures. This result is also characterized by nondenaturing PAGE.

关 键 词：DNA 选择性组装 PH 退火方式 Hoogsteen氢键 

分 类 号：Q523[生物学—生物化学] Q71