野核桃枝枯病菌拮抗细菌的筛选及鉴定  

Screening and Identification of the Antagonistic Bacteria against Wild Walnut Branch Rot

作　　者：王敏 赵颖[1] 蔡桂芳 王雪萍 张志东[3] 马荣[1] WANG Min;ZHAO Ying;CAI Gui-fang;WANG Xue-ping;ZHANG Zhi-dong;MA Rong(College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;Forestry Technology Extension Center of Changji Prefecture,Changji 831100,China;Institute of Mirobiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091)

机构地区：[1]新疆农业大学林学与园艺学院,乌鲁木齐830052 [2]昌吉州林业技术推广中心,昌吉831100 [3]新疆农业科学院微生物应用研究所,乌鲁木齐830091

出　　处：《新疆农业大学学报》2019年第4期261-266,共6页Journal of Xinjiang Agricultural University

基　　金：‘十三五’国家重点研发计划项目(2016YFC05011501)。

摘　　要：为得到对野核桃(Juglans regia L.)枝枯病有效的生防菌株,本研究采用平板对峙培养法筛选出对野核桃枝枯病菌(Juglanconis juglandina)具有拮抗作用的细菌菌株,对其进行形态学观察和16S rDNA序列同源性分析;观察拮抗细菌对菌丝生长的影响,分析该拮抗菌株的抑菌机制。结果表明,菌株QB002对野核桃枝枯病菌(J.juglandina)的生长抑制率为88.6%,同时对苹果腐烂病菌(Cytospora mali,C.salicicola)、枣果黑斑病菌(Alternaria alternata)、李褐腐病菌(Monilinia fructicola)都有一定抑制作用。该菌为革兰氏阳性细菌,在NA培养基上单菌落呈淡黄色,形状不规则,中间有褶皱,不透明,表面不光滑。结合形态特征及16S rDNA序列,明确菌株QB002为枯草芽孢杆菌(Bacillus subtilis),该菌能使供试病原菌菌丝畸形,从而抑制其生长。In order to obtain a biocontrol strain that was effective against wild walnut branch rot,this study used flat confrontation culture method to screen bacterial strains that antagonize Juglanconis juglandina.After that,morphology observation and homology analysis of 16S rDNA sequence were performed;The effects of antagonistic bacteria on mycelial growth were observed,and the antibacterial mechanism of the antagonistic strain was analyzed.The results showed that the growth inhibition rate of strain QB002 against J.juglandina was 88.6%,and meanwhile,it had a certain inhibitory effect on Cytospora mali,C.salicicola,Alternaria alternata,and Monilinia fructicola.The bacterium was a Gram-positive bacterium,and the single colony on the NA medium was light yellow,irregular in shape,wrinkled in the middle,opaque,and the surface was not smooth.According to morphological characteristics and 16S rDNA sequence,it was confirmed that the strain QB002 was Bacillus subtilis,which could make the mycelium of the pathogen deformed,thus inhibiting its growth.

关 键 词：野核桃枝枯病 拮抗细菌 筛选鉴定 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]