大叶糖胶树吲哚生物碱化学成分研究  

Indole alkaloids from Alstonia macrophylla

作　　者：张金苹 闫慧娇 王亚兰 王志伟[2] 王晓静[1,3,4] ZHANG Jin-ping;YAN Hui-jiao;WANG Ya-lan;WANG Zhi-wei;WANG Xiao-jing(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250200,China;Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 2500140,China;Institute of Materia Medica,Shandong Academy of Medical Sciences;Key Laboratory for Biotech-Drugs Ministry of Health,Key Laboratory forRare-Uncommon Diseases of Shandong Province,Jinan 2500620,China)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,济南250200 [2]山东省分析测试中心齐鲁工业大学(山东省科学院),济南250014 [3]山东省医学科学院药物研究所 [4]国家卫生部生物技术药物重点实验室山东省罕少见病重点实验室,济南250062

出　　处：《天然产物研究与开发》2020年第4期622-626,共5页Natural Product Research and Development

基　　金：国家自然科学基金(31700290)。

摘　　要：为研究大叶糖胶树(Alstonia macrophylla)化学成分及生物活性。采用正相硅胶、葡聚糖凝胶、制备液相等色谱技术,对大叶糖胶树中的化学成分进行了分离纯化,共分离得到6个化合物,通过NMR、MS等波谱方法将其结构鉴定为vallesamine(1)、picrinine(2)、khasuanine A(3)、scholaricine(4)、echitamine(5)、strictosamide(6)。化合物3和6首次从该属植物中分离得到,化合物1、2、4、5为首次从该植物中分离得到。所有化合物测试了对HCT-116人结肠癌细胞和DLD-1人结直肠腺癌上皮细胞的细胞毒性,结果显示化合物2和4具有一定的细胞毒性,化合物4对DLD-1人结直肠腺癌上皮细胞的细胞毒性较为明显(IC50 =16.7μg/mL),其余化合物均未显示出生物活性。In order to investigate the chemical constituents and biological activities of Alstonia macrophylla,six monoterpenoid indole alkaloids,namely vallesamine(1),picrinine(2),khasuanine A(3),scholaricine(4),echitamine(5),strictosamide(6) were isolated from leaves and twigs of Alstonia macrophylla by repeated column chromatography over silica gel,Sephadex G and Prep-HPLC.Their structures were elucidated based on NMR and MS spectroscopic data.Compounds 3 and 6 were firstly obtained from this genus,and compounds 1,2,4 and 5 were isolated from this plant for the first time.All compounds were evaluated the cytotoxicity of HCT-116 and DLD-1.Among them,compounds 2 and 4 had certain cytotoxicity,and compound 4 showed DLD-1 inhibitory activity with an IC50 value of 16.7 μg/mL.

关 键 词：大叶糖胶树 吲哚生物碱 生物活性 化学成分 

分 类 号：R284.1[医药卫生—中药学]