迷迭香酸抗动脉粥样硬化作用研究  

Rosmarinic acid attenuates atherosclerosis

作　　者：林晓琳 张凌云 LIN Xiao-lin;ZHANG Ling-yun(Department of Clinical Medicine,Binzhou Medical University;Department of Internal Medicine,Yantai Yuhuangding Hospital of Laishan Branch,Yantai 264003,China;Department of Endocrinology,Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264100,China)

机构地区：[1]滨州医学院临床医学院 [2]烟台毓璜顶医院莱山分院内科,烟台264003 [3]滨州医学院烟台附属医院内分泌科,烟台264100

出　　处：《天然产物研究与开发》2020年第4期652-658,688,共8页Natural Product Research and Development

摘　　要：本研究主要研究迷迭香酸是否能改善动脉粥样硬化(AS)。采用平行板流动腔模拟低剪切力(LSS)建立内皮细胞功能紊乱模型,使用DHE和DAF-FM DA探针检测细胞中ROS和NO的活性,分别使用qPCR法和Western blotting检测细胞中ICAM-1和VCAM-1的mRNA和蛋白表达来反应细胞炎症反应。采用高脂饲料(HCD)喂食斑马鱼建立斑马鱼AS模型,并在荧光显微镜下观察AS斑马鱼的脂质蓄积、炎症和氧化应激。结果发现,LSS诱导严重的内皮细胞功能紊乱,主要表现为:内皮细胞中ROS活性、ICAM-1和VCAM-1的mRNA和蛋白表达显著升高,而NO的活性显著降低。迷迭香酸的治疗可以有效改善LSS诱导的这些内皮细胞功能紊乱现象。结果也发现,HCD显著增加斑马鱼低剪切力处血管中的脂质蓄积,同时也诱导斑马鱼发生氧化应激和炎症反应。迷迭香酸治疗可以显著改善HCD诱导的这些AS症状。综上所述,迷迭香酸可以有效改善AS,并且其治疗AS的作用可能与其改善LSS诱导的内皮细胞功能紊乱作用有关。This study aimed to investigate the effectiveness of anti-atherosclerosis of rosmarinic acid(RosA).This study used a parallel flow chamber to establish low shear stress(LSS)-induced endothelial(EC) dysfunction model.This study exposed EC to LSS(3 dyn/cm^2) for 30 min and treated it by oridonin.the ROS and NO levels in EC were measured by DHE and DAF-FM DA assay.The mRNA and protein expression of ICAM-1 and VCAM-1 were assayed by qPCR and Westen blotting.This study fed zebrafish with high-cholesterol diet(HCD) to establish a zebrafish atherosclerosis(AS) model,and observed lipid accumulation and inflammation by a fluorescence microscope.Results found that RosA improved LSS-induced EC dysfunction,and inhibited HCD-induced lipid accumulation,oxidative stress and inflammation.Results indicated that RosA may be used for the treatment of AS,and primarily exhibited its protective role through improving LSS-induced EC dysfunction.

关 键 词：迷迭香酸 动脉粥样硬化 内皮细胞功能紊乱 斑马鱼 低剪切力 

分 类 号：R965[医药卫生—药理学]