响应面法优化乌骨藤中白桦脂酸提取工艺  

Extraction Optimization of Betulinic Acid from Marsdenia tenacissima Using Response Surface Methodology

作　　者：石玲[1] 王泽锋[1] 张薇[1] 闵勇[1] SHI Ling;WANG Ze-feng;ZHANG Wei;MIN Yong(College of Science,Honghe University,Mengzi 661199,Yunnan China)

机构地区：[1]红河学院理学院,云南蒙自661199

出　　处：《亚热带植物科学》2020年第2期93-98,共6页Subtropical Plant Science

基　　金：云南省教育厅项目(2020J0672);云南省科技厅项目(2013FZ120)。

摘　　要：为优化乌骨藤(Marsdenia tenacissima)中白桦脂酸的提取工艺,在单因素实验的基础上,采用Box-Behnken中心组合设计方法,研究超声功率、提取时间、料液比及提取次数等因素及其交互作用对白桦脂酸提取率的影响。利用Design Expert 8.06软件对数据进行回归分析,得到二次多项式回归方程的预测模型,并通过响应曲面优化方法得到白桦脂酸最佳提取工艺。结果表明,乌骨藤中白桦脂酸的最佳提取工艺为超声功率150 W,提取时间20 min,料液比(g/mL)为1:25,提取2次,白桦脂酸提取率预测值为0.314%,验证值为0.311%。Response surface methodology was used to optimize the extraction technology of betulinic acid from Marsdenia tenacissima.Based on single-factor experiments,ultrasonic power,extraction duration,material-liquid ratio,and times of extraction were selected as influencing factors during extraction.The experiment mathematical model was arranged according to Box-Behnken central composite experiment design,which studied each variable and their interactions on the extraction yield of betulinic acid.The quadratic multinomial model on the extraction yield of betulinic acid and each influence factor of extraction betulinic acid from M.tenacissima was established by using the Design-Expert software.And the optimum conditions were as follows:ultrasonic power 150 W,material-liquid ratio 1:25,extraction duration 20 min and extraction twice.The predicted value of extraction yield of betulinic acid was 0.314%under the optimum conditions,and the experimental value was 0.311%.

关 键 词：乌骨藤 白桦脂酸 响应曲面法 超声辅助提取 

分 类 号：R931.6[医药卫生—生药学]