复方半边莲口服液对乳腺癌荷瘤小鼠抗肿瘤免疫的影响  

Effect of compound oral liquid of Lobelia chinensis Lour on anti-tumor immunity in breast cancer-bearing mice

作　　者：夏雪竹 董永强 刘翠红[2] 管涛[3] 陈占功 XIA Xue-zhu;DONG Yong-qiang;LIU Cui-hong;GUAN Tao;CHEN Zhan-gong(Department of Pharmacy,1.Beijing Hospital of Traditional Chinese Medicine,Beijing 100010;Beijing You'an Hospital Affiliated to Capital Medical University,Beijing 100069;National Geriatric Center of Beijing Hospital,Beijing 100730,China)

机构地区：[1]北京中医医院药学部,北京100010 [2]首都医科大学附属北京佑安医院药学部,北京100069 [3]北京医院国家老年医学中心药学部,北京100730

出　　处：《川北医学院学报》2020年第2期203-206,共4页Journal of North Sichuan Medical College

摘　　要：目的:探究复方半边莲口服液对乳腺癌荷瘤小鼠的肿瘤抑制和改善肿瘤免疫的作用。方法:将24只BALB/C雌鼠原位接种4T1乳腺癌细胞,待瘤体体积超过40 mm 3时,随机分成对照组、复方半边莲口服液低、中、高剂量组,连续给药。每天测量荷瘤小鼠肿瘤大小,观察复方半边莲口服液对小鼠瘤体体积的影响;连续给药30 d后,处死荷瘤小鼠,通过测量不同组处理后荷瘤小鼠肿瘤重量,计算复方半边莲口服液各剂量组的抑瘤率;将肿瘤组织剪碎,经胶原酶处理成单细胞,并分离其中的淋巴细胞,使用流式分析仪检测肿瘤微环境中淋巴细胞的比例。结果:与对照组相比,复方半边莲口服液中、高剂量组于给药第10天、20天瘤体体积均降低(P<0.05),给药30 d,低、中、高剂量组瘤体体积均明显降低(P<0.01);低、中、高剂量组肿瘤重量明显降低(P<0.01),抑瘤率分别为17.95%、28.77%、49.43%;低、中、高剂量组CD3+T细胞、CD4+T细胞比例明显升高(P<0.01),中、高剂量组CD8+T细胞比例明显升高(P<0.01),而低、中、高剂量组调节性T细胞比例明显降低(P<0.01)。结论:复方半边莲口服液能够抑制乳腺癌荷瘤小鼠肿瘤的生长,增强荷瘤小鼠抗肿瘤免疫功能,值得临床推广应用。Objective:To examine the anti-tumor activity and immune-regulatory effect of compound oral liquid of Lobelia chinensis Lour in mice.Methods:24 BALB/C female mice were inoculated into 4T1 breast cancer cells in situ.When the tumor volume exceeded 40 mm 3,24 tumor-bearing mice were randomly divided into control group and compound oral liquid of Lobelia chinensis Lour low,medium and high dose groups.The tumor size of tumor-bearing mice was measured every day,and the anti-tumor effect of compound oral liquid of Lobelia chinensis Lour solution to the tumor volume of mice was observed.After 30 days of continuous administration,the tumor-bearing mice were sacrificed.And then,the tumors were weighed and measured as inhibition rate of each treated group.The tumor tissues were cut,treated with collagenase into single cells,and the lymphocytes were isolated,and the proportion of lymphocytes in the tumor microenvironment was detected by flow.Results:In terms of tumor volume,compared with the control group,the volume of tumor in the medium and high dose groups of compound oral liquid of Lobelia chinensis Lour were decreased on the 10 d and 20 d after administration(P<0.05).On the 30 d after administration,all the tumor volumes in treated group were significantly decreased(P<0.01).The tumor weight in the low,medium and high dose groups was significantly reduced(P<0.01),and the tumor inhibition rates were 17.95%,28.77%,and 49.43%,respectively.The proportion of CD3+T cells and CD4+T cells in the low,medium and high dose groups increased significantly(P<0.01).The proportion of CD8+T cells in the medium and high dose groups increased significantly(P<0.01),the proportion of regulatory T cells in the low,medium and high dose groups decreased significantly(P<0.01).Conclusion:Compound oral liquid of Lobelia chinensis Lour can inhibit the growth of tumor in breast cancer-bearing mice and enhance the anti-tumor immunity of tumor-bearing mice,which is worthy of clinical application.

关 键 词：复方半边莲口服液 乳腺癌 抑瘤率 肿瘤微环境 抗肿瘤免疫 

分 类 号：R285.5[医药卫生—中药学]