CRISPR/Cas9基因编辑技术在重要猪病毒病防控中的研究与应用  

Research Progress and Application of CRISPR/Cas9 Gene Editing Technology in Prevention and Control of Important Swine Virus Diseases

作　　者：戴学宇 张乾义[1] 徐璐[1] 赵启祖[1] 王琴[1] 夏应菊 DAI Xueyu;ZHANG Qianyi;XU Lu;ZHAO Qizu;WANG Qin;XIA Yingju(National/OIE Reference Laboratory for Classical Swine Fever,China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]中国兽医药品监察所国家/OIE猪瘟参考实验室,北京100081

出　　处：《畜牧兽医学报》2020年第5期943-951,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2018YFD0500801)。

摘　　要：规律成簇的间隔短回文重复序列系统(clustered regularly interspaced short palindromic repeat,CRISPR)是一种广泛存在于古细菌和细菌中,由RNA介导在Cas蛋白协助下发挥作用的获得性免疫系统,目前,已发现的CRISPR系统中以CRISPR/Cas9应用最为广泛,本文主要对CRISPR/Cas9系统的基本原理和研究进展进行概述,着重介绍其在重要猪病毒病防控中的应用,包括改造宿主和改造病毒两方面,该技术为研究病毒致病机制、新型疫苗研发以及抗病育种研究等提供了强有力的工具,对疫病的控制有着深远的影响。Clustered regularly interspaced short palindromic repeat(CRISPR)is an acquired immune system that exists widely in archaea and bacteria,mediated by RNA and assisted by Cas protein.It has been found that the CRISPR/Cas9 was the most widely used CRISPR system.This review mainly summarizes the basic principle and research progress of the CRISPR/Cas9 system,and highlights its application in the prevention and control of important swine virus diseases,including host modification and virus modification.This technology provides a powerful tool for the study of virus pathogenic mechanism,next generation vaccine development and disease resistance breeding.It has a far-reaching impact on the control of the diseases.

关 键 词：CRISPR/Cas9 猪病防控 新型疫苗 抗病育种 

分 类 号：Q78[生物学—分子生物学] S858.285.3[农业科学—临床兽医学]