健康人血红蛋白紫外可见吸收光谱和FTIR光谱  

UV-Visible Absorption Spectra and FTIR of Hemoglobin of Healthy People and It Spectroscopic Analysis

作　　者：丘家杵 阮萍 雍军光 冯博华 黄代政[5] 沈洪涛[6] QIU Jia-chu;RUAN Ping;YONG Jun-guang;FENG Bo-hua;HUANG Dai-zheng;SHEN Hong-tao(Department of Occupational and Environmental Health,Guangdong Pharmaceutical University,Guangzhou 510310,China;Department of Biomedical Engineering,Guangdong Pharmaceutical University,Guangzhou 510006,China;Department of Endocrinology,The Affiliated Outpatient Department of Guangdong Pharmaceutical University,Guangzhou 510235,China;Guangdong Province Engineering&Technology Research Center for Medical 3D Printer and Personalized Medicine,Guangzhou 510006,China;Department of Biomedical Engineering,Guangxi Medical University,Nanning 530021,China;College of Physics and Technology,Guangxi Normal University,Guilin 541001,China)

机构地区：[1]广东药科大学劳动卫生与环境卫生学系,广东广州510310 [2]广东药科大学生物医学工程系,广东广州510006 [3]广东药科大学附属门诊部,广东广州510235 [4]广东省医药3D打印机及个性化医疗工程技术研究中心,广东广州510006 [5]广西医科大学生物医学工程系,广西南宁530021 [6]广西师范大学物理科学与技术学院,广西桂林541001

出　　处：《光谱学与光谱分析》2020年第5期1425-1430,共6页Spectroscopy and Spectral Analysis

基　　金：广东省科技计划项目(2016A020215162);国家自然科学基金项目(11765004,81860604)资助。

摘　　要：为了探究健康人固体血红蛋白(hemoglobin,Hb)的紫外可见吸收光谱和傅里叶变化红外光谱的谱学特征,将21~80岁健康人分成四个年龄阶段组,采用葡聚糖层析法高度纯化新鲜血液红细胞内的Hb,利用冷冻干燥法获得结构稳定的Hb固体,测定四组样本的Hb紫外可见吸收光谱和FTIR光谱;利用OMNIC 8.0软件的QC比较分析功能、全光谱特征分析和二级结构分析首次归纳得出健康人Hb的紫外光谱及FTIR光谱。分析显示样本间,四组间Hb紫外可见吸收光谱和FTIR光谱高度相同,FTIR光谱间的匹配度高达95%;所有样本Hb的紫外光谱均有5个特征吸收峰,吸收峰所在位置的吸光度、波长相同;FTIR光谱均有13个特征吸收峰,吸收峰所在位置的吸光度、波数是相同的;Hb紫外吸收光谱显示健康人Hb拥有相同的三级结构,FTIR光谱的特征吸收带AmideⅠ带二阶导数谱匹配度高达99%,其二级结构均由11个子峰构成,组成则以α-螺旋为主。研究得出健康人Hb的紫外可见吸收光谱和FTIR光谱,并通过初步分析得到Hb的光谱学特征和结构特点,有助于研究相关疾病。This study focused on the UV-Visible absorption spectra and the Fourier-infrared spectroscopy of hemoglobin(Hb)in healthy humans and analyzed its spectral characteristics to obtain the UV-Visible absorption spectrum and FTIR of solid Hb with a stable structure in healthy humans.The 21~80-year-old subjects were divided into 4 groupstocollect blood samples.Hemoglobin was purified by G-75 glucan after centrifugation,Structure-stable Hb solids were obtained by the freeze-drying method,and Hb of four groups were determined Ultraviolet-visible absorption spectroscopy and FTIR spectroscopy.QC comparative analysis,full-spectral characterization and secondary structure analysiswere used to obtain the UV and FTIR spectra of healthy human Hb.The analysis showed that the Hb UV-visible absorption spectra and FTIR spectra were the same between the four groups.The matching between the QC and the FTIR spectra was as high as 95%.The UV spectra of all the samples had five characteristic absorption peaks,and the absorption peaks absorbance and wavelength of the position were same.The FTIR spectrum had 13 characteristic absorption peaks,and the absorbance and wave number of the position of the absorption peak between different samples were same;preliminary analysis of Hb ultraviolet absorption spectrum showed that healthy human Hb had the same tertiary structure.The characteristic absorption band of FTIR spectrum AmideⅠband had second derivative spectrum matching degree of up to 99%,and its secondary structure was composed of 11 sub-peaks,and the composition was mainlyα-helix.The UV-Vis absorption spectroscopy and FTIR spectroscopy of the structurally stable solid human Hb were obtained.The spectroscopy and structural characteristics of Hb were obtained through preliminary analysis,which was helpful to study related diseases.

关 键 词：健康人 血红蛋白 紫外光谱 FTIR 参照光谱 

分 类 号：O657.3[理学—分析化学]