β溶血表型摩根摩根菌的鉴定及系统发育树构建  

Identification and phylogenetic tree construction of beta hemolytic Morganella morganii

作　　者：贾琴妹 满宝华 JIA Qinmei;MAN Baohua(Department of Clinical Laboratory,The third People's Hospital of Yunnnan Province,KunMing,Yunnan,China,650011)

机构地区：[1]云南省第三人民医院检验科微生物室,云南昆明650011

出　　处：《分子诊断与治疗杂志》2020年第3期314-318,共5页Journal of Molecular Diagnostics and Therapy

基　　金：云南省科技厅应用基础研究联合专项资金项目(201501UC00015)。

摘　　要：目的对β溶血表型摩根摩根菌进行准确的鉴定和系统发育分析。方法常规方法分离培养菌株;先后采用VITEK-2 Compact全自动微生物鉴定仪、机制辅助激光解析离子-飞行时间质谱仪(MALDI-TOF-MS)、16S rDNA测序3种方法对菌株进行鉴定并验证其结果的正确性;基于16S rDNA的同源性分析和系统发育树构建;并对溶血基因的检测。结果采用16S rDNA序列同源性分析结合构建系统发育树分析,鉴定菌株(标本编号:U1901)与摩根摩根菌摩根亚种NBRC 3848形成一个分支,同源性和可信度均达99.99%,同时验证了MALDI-TOF-MS的鉴定结果比VITEK-2 Compact全自动微生物鉴定仪准确及时。检测出新表型摩根摩根菌(β溶血表型)。结论16S rDNA序列同源性分析与系统发育树构建相结合,准确鉴定出新型表型的摩根摩根菌(β溶血表型)。MALDI-TOF-MS在病原微生物检测和鉴定中具有快速、准确等优点,可为临床治疗提供快速的诊断依据。Objective To identify beta hemolytic Morganella morganii and perform the phylogenetic analysis. Methods The bacterial strain was cultured and isolated by conventional methods. Three methods,VITEK-2 Compact automatic microbial identification instrument,Matrix-assisted Laser Desorption/Ionozation-time of Flight Mass Spectrometry(MALDI-TOF-MS)and 16 SrDNA sequencing,were used to identify the strain and validatethe the result. Based on homology analysis of 16 S rDNA,a phylogenetic tree was constructed;and hemolytic genes were detected. Results Using 16 srDNA sequence homology analysis combined with phylogenetic tree construction,the identification strain(Sample No.:U1901)formed a separate branch with Morganella morganii subsp.morganii NBRC 3848,the homology and credibility were 99.99%. The identification result of MALDI-TOF-MS was more accurate and timely than that of VITEK-2 Compact automatic microbial identification instrument. A new phenotype of Morganella morganii(beta hemolytic phenotype) was detected. Conclusion The combination of 16 S rDNA sequence homology analysis and phylogenetic tree construction accurately identified a new phenotype of Morganella morganii(beta hemolytic phenotype)MALDI-TOF-MS has the advantages of rapid and accurate in the detection and identification of pathogenic microorganisms,which can provide a rapid diagnostic basis for clinical treatment.

关 键 词：摩根摩根菌 β溶血 16S RDNA 系统发育树 

分 类 号：R446.5[医药卫生—诊断学]