血链球菌细菌素对伴放线聚集杆菌和白色念珠菌生物膜作用的研究  

Role of Streptococcus Sanguis Bacteriocin on Regulating the Activity of Biofilms of Aggregatibacter Actinomycetemcomitans and Candida Albicans

作　　者：吴音璇 马晟利[2] 葛文玉 李慧[2] 吴丽春[2] 王馨[2] 李春梅[2] WU Yinxuan;MA Shengli;GE Wenyu;LI Hui;WU Lichun;WANG Xin;LI Chunmei(Jiamusi University, Jiamusi 154002, China;Heilongjiang Provincial Hospital, Harbin 150036, China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154002 [2]黑龙江省医院,黑龙江哈尔滨150036

出　　处：《口腔医学研究》2020年第4期360-364,共5页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金(编号:H2015086)。

摘　　要：目的:研究血链球菌细菌素分别对单独培养及混合培养的伴放线聚集杆菌(A.a)和白色念珠菌(C.a)生物膜的最小抑菌浓度(MIC)。利用激光共聚焦显微镜研究血链球菌细菌素对A.a及混合培养的A.a和C.a生物膜活性的影响。方法:通过低温高速离心,细胞破碎,盐析等方法提取血链球细菌素—血链素。采用二倍稀释法,测定血链素对单独和混合培养的A.a及C.a的MIC;血链素作用于A.a及混合培养的A.a和C.a后,利用激光共聚焦显微镜分别在2 h、6 h、12 h、24 h、48 h、72 h观察生物膜活性的变化。结果:血链素对单独培养的A.a的MIC为0.25 g/L;血链素对单独培养的C.a的MIC为0.125 g/L;血链素对混合培养的A.a及C.a的MIC为0.5 g/L。通过激光共聚焦显微镜观察,血链素对A.a生物膜活性有较强的抑制作用,最显著的时间在72 h;血链素对混合培养的A.a及C.a生物膜活性同样具有较强的抑制作用,最显著的时间在48 h。结论:血链素对A.a及混合培养的A.a及C.a生物膜活性均有抑制作用。Objective:To study the MIC of streptococcus sanguis bacteriocin on the biofilm of A.a and C.a.and to study the effects of Streptococcus sanguis bacteriocin on the biofilm activity of A.a and mixed cultured A.a and C.a.by laser confocal microscopy.Methods:Through low temperature high speed centrifugation,cell fragmentation,salting out,and other methods,Streptococcus sanguis bacteriocin was extracted.The MIC of A.a and mixed cultured A.a and C.a were determined by double dilution method.The biofilm activity was observed after 2h,6h,12h,24h,48h,and 72h by laser confocal microscope after the action of Streptococcus sanguis bacteriocin on A.a and mixed cultured A.a and C.a.Results:The MIC of separate A.a was 0.25 g/L.The MIC of separate C.a was 0.125 g/L.The MIC of the mixed cultured A.a and C.a was 0.5 g/L.The results of laser confocal microscope showed that the biofilm activity of A.a was strongly inhibited by streptococcus sanguis bacteriocin.The most significant time was 72 h.The biofilm activity of mixed culture A.a and C.a were also strongly inhibited by Streptococcus sanguis bacteriocin.The most significant time was 48 h.Conclusion:Streptococcus sanguis bacteriocin inhibited the biofilm activity of separate A.a and the mixed cultured A.a and C.a.

关 键 词：血链球菌细菌素 伴放线聚集杆菌 白色念珠菌 生物膜 

分 类 号：S85[农业科学—兽医学]