甲状旁腺激素干预兔下颌骨髁突高位骨折游离复位后的软骨愈合  

Effect of parathyroid hormone on cartilage healing in a rabbit model of mandibular condylar fracture

作　　者：谢柳琴 黄志才 王光素 张国兴 李杜晨晖 李骁 唐正龙 Xie Liuqin;Huang Zhicai;Wang Guangsu;Zhang Guoxing;Li-Du Chenhui;Li Xiao;Tang Zhenglong(Stomatological Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州医科大学附属口腔医院,贵州省贵阳市550004

出　　处：《中国组织工程研究》2020年第32期5114-5121,共8页Chinese Journal of Tissue Engineering Research

基　　金：贵州省科学技术基金(黔科合基础[2017]1151),项目负责人:唐正龙。

摘　　要：背景:下颌骨髁突高位骨折常伴有软骨的损伤,同时由于附着于髁突的肌肉发生撕脱而成为游离骨块,如何在加快骨愈合同时加快软骨的愈合一直是临床上面临的困难和挑战。目的:探讨甲状旁腺激素在兔髁突高位骨折游离复位后对髁突软骨愈合的影响。方法:将48只新西兰大耳兔建立髁突高位骨折游离复位固定实验模型,随机分为实验组及对照组,每组24只。术后实验组隔日皮下注射甲状旁腺激素20μg/kg,对照组注射生理盐水1 mL;分别在术后1,2,3,4周时处死,取下颌骨髁突标本行组织形态学研究,免疫组织化学和PCR检测髁突软骨中Sox9和基质金属蛋白酶13的表达。实验方案经贵州医科大学动物实验伦理委员会批准(批准号为1700456)。结果与结论:①番红O-固绿染色及苏木精-伊红染色显示实验组中软骨骨折区的软骨细胞数量及软骨基质沉积都较对照组多;②免疫组织化学显示各时期的实验组Sox9因子的表达强度均强于对照组(术后1-3周P<0.05);实验组基质金属蛋白酶13表达在术后1,2,3周显著低于对照组(P<0.05);③各时期实验组Sox9mRNA的表达量均高于对照组(术后1-3周P<0.05);实验组中基质金属蛋白酶13 mRNA的表达量低于对照组(术后1-3周P<0.05);④结果说明,间歇性皮下注射甲状旁腺激素,可上调Sox9、抑制早期基质金属蛋白酶13的表达,促进间充质干细胞向软骨转化,加快修复软骨损伤。BACKGROUND:A high fracture of the mandibular condyle is often accompanied by cartilage damage.At the same time,the muscle attached to the condyle is avulsed and becomes a free bone mass.How to speed up the concurrent healing of cartilage during bone healing has always been a clinical difficulty and challenge.OBJECTIVE:To investigate the effect of parathyroid hormone on the healing of condylar cartilage in rabbits with high condylar fracture after free reduction.METHODS:An experimental model of free reduction and fixation of condylar fracture was established in 48 New Zealand big-eared rabbits,which were randomly divided into experimental group and control group(n=20per group).The experimental group was injected with parathyroid hormone(20μg/kg)subcutaneously every other day,and the control group was injected with 1 m L of normal saline.The animals were sacrificed at 1,2,3,and 4 weeks postoperatively.The mandible condyle was histologically observed.Immunohistochemistry staining and PCR were used to detect the expression of Sox9 and matrix metalloproteinase 13 in the condylar cartilage.The study protocol was approved by the Animal Experimental Ethics Committee of Guizhou Medical University(approval No.1700456).RESULTS AND CONCLUSION:The results of safranine O-fast green staining and hematoxylin-eosin staining indicated that there were more chondrocytes and cartilage matrix deposition in the experimental group than the control group.In the immunohistochemistry,the average absorbance of Sox9 in the experimental group was significantly higher than that in the control group within 1-3 postoperative weeks(P<0.05).The average absorbance of matrix metalloproteinase 13 in the experimental group was lower than that in the control group within 1-3 postoperative weeks(P<0.05).The expression of Sox9 m RNA in the experimental group was significantly higher than that in the control group within 1-3 postoperative weeks,P<0.05).The expression of matrix metalloproteinase 13 m RNA in the experimental group was significantly lower t

关 键 词：骨 骨折 髁突游离骨折 甲状旁腺激素 基质金属蛋白酶13 SOX9 模型 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]