免疫性卵巢早衰模型鼠制备的时间  

Time for preparing a mouse model of autoimmune premature ovarian failure

作　　者：田海清[1] 张于念 张和江 范莹露 腊晓琳[1] Tian Haiqing;Zhang Yunian;Zhang Hejiang;Fan Yinglu;La Xiaolin(Center for Reproductive Medicine,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院生殖医学中心,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2020年第32期5168-5172,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金(2017D01C318),项目负责人:范莹露。

摘　　要：背景:研究已经证实抗透明带抗体可以加速卵母细胞的破坏和耗竭而致卵巢早衰。目的:探讨以透明带3多肽(pZP3)诱导BALB/c小鼠建立免疫性的卵巢早衰模型的时间。方法:6-8周龄的健康雌性BALB/c小鼠30只,随机分为免疫实验组(20只)和空白对照组(10只)。实验组小鼠首次免疫注射时间为0周,给予0.15 mL免疫试剂注射双足底及下腹部皮下,每日晨阴道脱落细胞涂片观察小鼠动情周期的变化;2周后给予0.15mL免疫强化试剂皮下注射相同部位,第4周和第6周的第1天,分别注射免疫试剂或免疫强化试剂各1次(交替注射)。在每次注射前采血用酶联免疫法测小鼠血清性激素;最后观察小鼠卵巢组织及子宫形态。结果与结论:①实验组小鼠初次免疫注射及注射后2周,2组间血清促卵泡生长激素及雌激素水平差异无显著性意义;注射后4周实验组血清雌激素水平明显低于对照组,但促卵泡生长激素水平差异无显著性意义;注射后6周实验组血清雌激素水平明显低于对照组(P<0.05),促卵泡生长激素水平明显高于对照组(P<0.01);②实验组小鼠卵巢间质纤维化程度较对照组明显,卵巢体积减小,子宫萎缩,小鼠原始卵泡、初级及次级卵泡数均显著低于对照组,闭锁卵泡数量高于对照组(P<0.05);③结论:75μg透明带3多肽诱导BALB/c小鼠,建立自身免疫性卵巢早衰疾病模型,免疫后6周即可达到良好的建模效果。BACKGROUND:Studies have confirmed that anti-zona zona antibodies can accelerate the destruction and depletion of oocytes,thereby causing premature ovarian failure.OBJECTIVE:To investigate the time of establishing autoimmune premature ovarian failure model in BALB/c mice induced by zona pellucida 3 peptides.METHODS:Thirty healthy female BALB/c mice(7-8 weeks)were randomly divided into immune experimental group(20 mice)and the control group(10 mice).In the experimental group,the mice were given immunization injection starting at 0 week,0.15 mL of immune reagent injected into the soles at both sides and the lowerabdomen.The vaginal exfoliated cell smears were observed every morning to observe the changes in the estrous cycle of the mice.After 2 weeks of injection,0.15 m L of immune enhancement agent was injected subcutaneously into the same site.On the first day of weeks 4 and 6,the immune agent or immune enhancement agent was injected alternatively.Blood samples were collected before each injection and the serum sex hormone level was measured by enzyme-linked immunosorbent assay.Finally,ovarian tissue and uterus morphology were observed.RESULTS AND CONCLUSION:There was no difference in serum follicle stimulating hormone and estrogen levels between the first immunization injection and 2 weeks after the injection.The serum estrogen level in the experimental group was significantly lower than that in the control group at 4 weeks after injection.The serum estrogen level of the experimental group was significantly lower than that of the control group at 6 weeks after injection(P<0.05),and meanwhile the serum follicle stimulating hormone level was significantly higher than that of the control group(P<0.01).The degree of ovarian interstitial fibrosis in the experimental group was more obvious than that in the control group.The ovarian volume decreased and the uterus atrophied in the experimental group.The number of primitive follicles,primary follicles and secondary follicles in mice was significantly lower in the experim

关 键 词：透明带3多肽 鼠 免疫性疾病 卵巢早衰 动物 模型 

分 类 号：R466[医药卫生—临床医学] R318