γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响  

Effects ofγ-irradiation and EDC/NHS modificationon property of collagen-chitosan scaffolds

作　　者：杜歌 魏莉 刘自双 武继民 陈子浩 田丰 DU Ge;WEI Li;LIU Zi-shuang;WU Ji-min;CHEN Zi-hao;TIAN Feng(Geriatric Rehabilitation Center,Beijing Rehabilitation Hospital of Capital Medical University,Beijing 100144,China;Instituteof Health Service Support Technology,Academy of Military Sciences,Tianjin 300161,China;Aviation and Nautical Medical Center,the Sixth Medical Center of PLA General Hospital,Beijing 100048,China)

机构地区：[1]首都医科大学附属北京康复医院老年康复中心,北京100144 [2]军事科学院卫勤保障技术研究所,天津300161 [3]中国人民解放军总医院第六医学中心航海航空医学中心,北京100048

出　　处：《材料工程》2020年第5期106-111,共6页Journal of Materials Engineering

基　　金：天津市应用基础与前沿技术研究计划资助项目(13JCZDJC33400)。

摘　　要：以5%梯度制备11组壳聚糖质量分数为0%~50%的胶原壳聚糖支架。使用γ辐射和EDC/NHS分别改性处理各组胶原壳聚糖支架,采用傅里叶变换红外光谱仪(FTIR)和扫描电镜(SEM)分析支架内部结构,利用吸水率、孔隙率、降解率和力学性能等指标对其性能进行检测,研究γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响。结果表明:γ辐射和EDC/NHS改性均能使胶原与壳聚糖产生交联,壳聚糖的加入改善了γ辐射对支架分子结构的损伤;EDC/NHS改性支架的微结构好于γ辐射支架;两种改性支架壳聚糖较优,质量分数均为25%;γ辐射和EDC/NHS改性均能使支架产生取向结构。With chitosan mass fraction changing from 0%to 50%by 5%,relative to collagen mass,the collagen-chitosan scaffolds were crosslinked byγ-irradiation and EDC/NHS.Fourier transform infrared spectroscopy(FTIR)and SEM were applied to analyze the structure.Water absorption rate,porosity,collagenase degradation and mechanical property were used to detect the properties.The effects ofγ-irradiation and EDC/NHS modification on the properties of scaffolds were studied.The results show that bothγ-irradiation and EDC/NHS modification induce crosslinking in collagen-chitosan scaffolds and chitosan greatly reduce the damage to molecular structure caused byγ-irradiation.The microstructure crosslinked byγ-irradiation is worse than those crosslinked by EDC/NHS.The optimal chitosan mass fraction is 25%for bothγ-irradiation and EDC/NHS.γ-irradiation and EDC/NHS scaffold modification can lead to orientation structure.

关 键 词：胶原壳聚糖支架 Γ辐射 EDC/NHS 交联 改性 

分 类 号：R318.08[医药卫生—生物医学工程]